Shortening of dioestrus in female dogs using cloprostenol sodium at different stages of dioestrus
J. Deepak, M. P. Unnikrishnan, C. Jayakumar, Hiron M. Harshan and K. D. John Martin
A study was conducted in 18 healthy, fertile, non-pregnant, dioestrous female dogs to evaluate the efficacy of PGF2α analogue (cloprostenol sodium) in shortening the dioestrus. Bitches that were diagnosed non-pregnant by trans-abdominal ultrasonography, on day 30 of breeding and their dioestrual stage confirmed by serum progesterone assay, were randomly allotted to three groups of six bitches each. Group I and II bitches were treated with cloprostenol sodium @ 2.5 µg/kg b. wt., subcutaneously, thrice at 48 h interval, beginning on day 30 and 45 of last breeding, respectively. Group III animals were assigned as control and were left untreated. Fifty days from first injection of cloprostenol, serum progesterone assay was carried out among the animals in Group I and II whereas the same was done among Group III bitches on day 90 after last breeding. Among Group I and II, mean serum progesterone concentrations were 0.93 ± 0.04 and 0.87 ± 0.01ng/ mL, respectively whereas the level was 1.72 ± 0.9 ng/mL among control group; highly significant difference (p<0.01) existed between control and treatment groups. The present study indicated that administration of PGF2α analogue during dioestrus enhances the pace of luteal regression among non-pregnant bitches.
Keywords: Dioestrus, PGF2α, luteolysis, non-pregnant, bitches