RESEARCH ARTICLES
Epidemiological investigation for brucellosis in dogs of Thrissur
K. Athira, V. H. Shyma, K. D. Justin, K. Vijayakumar and C. Jayakumar
doi:https://doi.org/10.51966/jvas.
Journal of Veterinary and Animal Sciences.2021. 52(4):389-392.
Author Details
K. Athira: MVSc scholar, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
V. H. Shyma: Assistant Professor, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
K. D. Justin: Assistant Professor, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
K. Vijayakumar: Professor and Head, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
C. Jayakumar: Assistant Professor, Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
Article History
Received: 14.03.2021, Accepted: 19.05.2021, Published online: 15.12.2021
Corrersponding author: K. Athira,
e-mail: athirasonukondath@gmail.com
Citation: Athira, K., Shyma, V. H., Justin, K. D., Vijayakumar, K. and Jayakumar, C. 2021. Epidemiological investigation for brucellosis in dogs of Thrissur. J. Vet. Anim. Sci. 52(4): 389-392. DOI: https://doi.org/10.51966/jvas.
Abstract
India is endemic to bovine brucellosis, and there is a high potential for transmission of disease from ruminants to dogs. A total of 18 bitches belonging to five different breeds at different stage of abortion (30 days to 65 days of gestation) were selected for this study. Majority of them were showing abortion (88.89 per cent) at 45 to 65 days of the gestation. Microscopic examination of Stamp stained smear obtained from the aborted foetal stomach contents revealed red coccobacillary organisms suggestive of Brucella spp.in three cases. RBPT on paired sera samples on day of presentation and three weeks after abortion showed agglutination within four minutes in five out of 18 female dogs. DNA extracted from the aborted tissues of a RBPT positive Labrador dog yielded amplicons of 193 base pair specific for Brucella spp. on PCR. The results obtained from this study stress the need for screening dogs for canine brucellosis in the current brucellosis surveillance and control programmes. Keywords: Bovine brucellosis, abortion, zoonotic disease, stamp staining, RBPT, PCR
