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In vitro maturation of goat oocytes selected using Brilliant cresyl blue staining
T. S. Arya, Amritha Aravind, R. S. Abhilash, C. Jayakumar and V. Babitha
doi: https://doi.org/10.51966/jvas.
Journal of Veterinary and Animal Sciences.2021.52 (4):405-408.
Author Details
T. S. Arya: M.V.Sc. scholar, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary & Animal Sciences, Mannuthy, Thrissur, Kerala – 680651 Kerala Veterinary and Animal Sciences University, India.
Amritha Aravind: Assistant Professor, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary & Animal Sciences, Mannuthy, Thrissur, Kerala – 680651 Kerala Veterinary and Animal Sciences University, India.
R. S. Abhilash: Assistant Professor, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary & Animal Sciences, Mannuthy, Thrissur, Kerala – 680651 Kerala Veterinary and Animal Sciences University, India.
C. Jayakumar: Assistant Professor and Head (i/c), Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary & Animal Sciences, Mannuthy, Thrissur, Kerala – 680651 Kerala Veterinary and Animal Sciences University, India.
V. Babitha: Assistant Professor, Department of Physiology, College of Veterinary & Animal Sciences, Mannuthy, Thrissur, Kerala – 680651 Kerala Veterinary and Animal Sciences University, India.
Article History
Received: 05.02.2021 Accepted: 09.03.2021 Published: 15.12.2021
Corresponding author: T. S. Arya,
e-mail: aryats777@gmail.com
Citation: Arya, T. S., Amritha, A., Abhilash, R. S., Jayakumar, C. and Babitha, V. 2021. In vitro maturation of goat oocytes selected using Brilliant cresyl blue staining. J. Vet. Anim. Sci. 52(4): 405-408. DOI: https://doi.org/10.51966/jvas.
Abstract
The present study was conducted to assess the developmental competence of goat oocytes selected using Brilliant cresyl blue (BCB) staining. Goat ovaries were collected from the slaughtered animals with unknown reproductive history. The oocytes retrieved by aspiration technique were selected based on morphology and subjected to BCB staining. Brilliant cresyl blue staining is based on the activity of glucose-6-phospahte dehydrogenase (G6PDH) enzyme synthesised by the oocytes. The cytoplasm remains blue in oocytes that have finished the growth phase (BCB+) while the growing oocytes remain colourless (BCB-). The stained and unstained oocytes were subjected to in vitro maturation separately to assess cumulus cell expansion index and polar body extrusion. A total of 206 culture grade oocytes were subjected to study, out of which, 76.75 ± 2.38 per cent of oocytes showed positive to BCB staining and 23.21 ± 2.38 per cent were negatively stained. Significantly higher maturation rate was observed in BCB+(92.89 ± 2.37%) oocytes than BCB-(29.72 ± 2.46%). The present study concluded that BCB staining can be used for selecting goat oocytes with good cytoplasmic maturation for further in vitro embryo production.
Key words: Goat oocytes, in vitro maturation, Brilliant cresyl blue
