Cryopreservation of Trypanosoma evansi
M. R. Saseendranath, J. Ramkrishna, M. John Edwin, M. C. John and R. Anandan

Journal of Veterinary and Animal Sciences.1992. 23:25-27.

Copyright: © 1992 M. R. Saseendranath, This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



T. evansi organisms were harvested from parasitaemic rats and cryopreserved with various preservatives. Tris-buffer was found to be superior to RPMI 1640 in preserving the parasites. The cryoprotectants glycerol afforded higher survival compared to DMSO.