IN VITRO CULTIVATION OF THE CELLS OF CARCINOMA OF THE MUCOSA OF THE ETHMOID
Madan Singh Karki and A. Rajan
Ethmoid carcinoma cells obtained from cattle and goats were grown in HBSS, TC 199 and Dulbecco's modified Eagle's medium with 10 to 20 per cent calf serum. The tumour cells obtained from cows failed to grow in all the media used. The tumour cells from goats grew and could be maintained for nine days. Subsequently the cells died even after subculturing and changing of the media. The possible role of endogenous infective agents, degenerated condition of the tumour tissue and absence of certain unknown factors required by the neoplastic cells for growth were attributed as factors responsible for failure.