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Phylogenetic analysis of Nilgiri langur using mitochondrial cytochrome B gene
K. V. Meganath, Muhasin Asaf, G. Radhika, George Chandy, M. Manoj, C. N. Dinesh, Jacob Alexander, E. M. Muhammed and P. M. Rojan
doi: https://doi.org/10.51966/jvas.2022.53.1.112-116
Journal of Veterinary and Animal Sciences.2022.53 (1): 112-116
Author Details
K. V. Meganath : M.V.Sc Scholar, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode – 673 576 Kerala Veterinary and Animal Sciences University, Kerala, India
Muhasin Asaf : Assistant Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode – 673 576 Kerala Veterinary and Animal Sciences University, Kerala, India
G. Radhika: Associate Professor and Head, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode – 673 576 Kerala Veterinary and Animal Sciences University, Kerala, India
George Chandy : Special Officer, Centre for Wildlife Studies, KVASU, Pookode
M. Manoj : Assistant Professor, Centre for Pig Production and Research, KVASU, Mannuthy
C. N. Dinesh : Associate Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode – 673 576 Kerala Veterinary and Animal Sciences University, Kerala, India
Jacob Alexander : Assistant Director, Zoological Gardens, Department of Museums and Zoos, Thiruvananthapuram
E. M. Muhammed : Assistant Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode – 673 576 Kerala Veterinary and Animal Sciences University, Kerala, India
P. M. Rojan : Assistant Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode – 673 576 Kerala Veterinary and Animal Sciences University, Kerala, India
Article History
Received: 21.06.2021 Accepted: 11.07.2021 Published: 31.03.2022
Corresponding author: K. V. Meganath
e-mail: meganathvet@gmail.com
Citation: Meganath, K. V., Muhasin, A., Radhika, G., George, C., Manoj, M., Dinesh, C. N., Jacob, A., Muhammed, E. M. and Rojan, P. M. 2022. Phylogenetic analysis of Nilgiri langur using mitochondrial cytochrome B gene. J. Vet. Anim. Sci. 53(1): 112-116.
DOI: https://doi.org/10.51966/jvas.2022.53.1.112-116
Abstract
Old world monkeys comprise 28 Langur species which belong to subfamily Colobinae under family Cercopithecidae. Nilgiri Langurs (Trachypithecus johnii) are endemic to the rain forests of the Western Ghats. The current study is an attempt at comparative phylogeny based on mitochondrial CYTB (mtCYTB) gene of Nilgiri Langur with other langurs. Faecal sample was collected from Nilgiri langur and the genomic DNA was isolated. The 1140 bp mitochondrial CYTB was amplified and sequenced using Sanger’s di-deoxy method. The amplified sequence along with the 27 sequences of Trachypithecus and Semnopithecus that were retrieved from the GenBank database were used for analysis. The phylogenetic tree was constructed by the maximum likelihood method in MEGA X. The analysis showed the clustering of Nilgiri langur with other langurs of the Semnopithecus sp. as a single clade.
Keywords: Mitochondria, Langur, CYTB, Trachypithecus, Semnopithecus, phylogeny
