RESEARCH ARTICLES
Tetracycline efflux pump genes in Escherichia coli from retail chicken in central Kerala
Binsy Mathew, C. Latha , B. Sunil, C. Sethulekshmi, and G. Radhika
doi: https://doi.org/10.51966/jvas.2022.53.2.163-169
Journal of Veterinary and Animal Sciences.2022.53(2):163-169.
Author Details
Binsy Mathew: Assistant Professor,Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680 651 Kerala Veterinary and Animal Sciences University, Kerala, India.
C. Latha: Professor and Head, Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680 651 Kerala Veterinary and Animal Sciences University, Kerala, India.
B. Sunil: Professor and Head, Department of Livestock Products Technology,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680 651 Kerala Veterinary and Animal Sciences University, Kerala, India.
C.Sethulekshmi: Assistant Professor,Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680 651 Kerala Veterinary and Animal Sciences University, Kerala, India.
G. Radhika: Associate Professor and Head, Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences, Pookode, Kerala, India.
Article History
Received:12.05.2021,Accepted:19.06.2021,Published: 30.06.2022
Corresponding author: Binsy Mathew
email: binsymathew@kvasu.ac.in
Citation: Binsy, M., Latha, C., Sunil, B., Sethulekshmi, C., and Radhika, G. 2022. Tetracycline efflux pump genes in Escherichia coli from retail chicken in central Kerala. J. Vet. Anim. Sci. 53(2): 163-169 DOI: https://doi.org/10.51966/jvas.2022.53.2.163-169
Abstract
The rearing of chicken in India has undergone a drastic change from backyard production to commercial intensive farming. This has led to the use of antibiotics in therapy, metaphylaxis and as growth promoters. Escherichia coli are commensals that inhabit the gut of man and animals. The detection of virulent pathotypes of E. coli in chicken is a huge threat to human health. The present study assessed 200 retail chicken sold in central Kerala for the presence of virulent E. coli and studied the tetracycline susceptibility followed by detection of tetA and tetB gene. E. coli was detected in 64 per cent of the samples. The virulence genes, eaeA and aggR were detected in 52.2 and 3.9 per cent of the E. coli isolates, respectively. Tetracycline resistance by antibiotic susceptibility testing (ABST) was found in 30.84 per cent of the virulent isolates. The tetracycline efflux pump protein coding genes, tetA and tetB were detected in 56.67 and 25 per cent, respectively. The detection of drug resistant bacteria is a threat to public health as tetracycline is classified as a highly important antibiotic in human medicine.
Keywords: E. coli, tetracycline resistance, tetA, tetB
