RESEARCH ARTICLES
Molecular detection of methicillin resistant Staphylococcus aureus associated with mastitis in goats
J. N. Bhagya, P. V. Tresamol , K. Vijayakumar , V. H. Shyma and M. Mini
doi: https://doi.org/10.51966/jvas.2022.53.2.189-194
Journal of Veterinary and Animal Sciences.2022.53(2):189-194
Author Details
J. N. Bhagya: MVSc Scholar,Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651 Kerala Veterinary and Animal Sciences University, Kerala, India
P. V. Tresamol: Professor , Department of Veterinary Epidemiology and Preventive Medicine,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651 Kerala Veterinary and Animal Sciences University, Kerala, India
K. Vijayakumar: Professor and Head,Department of Veterinary Epidemiology and Preventive Medicine,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651 Kerala Veterinary and Animal Sciences University, Kerala, India
V. H. Shyma: Assistant Professor,Department of Veterinary Epidemiology and Preventive Medicine,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651 Kerala Veterinary and Animal Sciences University, Kerala, India
M.Mini: Professor and head, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680 651 Kerala Veterinary and Animal Sciences University, Kerala, India
Article History
Received:30.06.2021,Accepted:27.10.2021,Published:30.06.2022
Corresponding author :J. N. Bhagya
email:jbhagya93@gmail.com
Citation: Bhagya J. N., Tresamol, P. V., Vijayakumar, K., Shyma, V. H. and Mini, M. 2022. Molecular detection of methicillin resistant Staphylococcus aureus associated with mastitis in goats. J. Vet. Anim. Sci. 53(2): 189-194 DOI: https://doi.org/10.51966/jvas.2022.53.2.189-194
Abstract
Antimicrobial resistance is an emerging concern in the world while combating with a wide range of pathogen associated with various disease conditions. It is considered as a silent pandemic nowadays. A constant monitoring and measures for controlling emergence of antimicrobial resistance are crucial in the current situation. The present study was conducted with the objective of detection of methicillin resistant Staphylococcus aureus (MRSA) associated with mastitis in goats. Milk samples were collected from 66 goats suffering from mastitis and screened for the presence of S. aureus. Conventional microbiological tests and species specific PCR were employed for the species confirmation. A total of 10 samples (15.15%) were found to be positive for S. aurues. Among the ten isolates of S. aureus obtained two isolates (3.03%) were found to harbour mecA gene suggestive of the presence of MRSA phenotype. All the isolates were negative for the presence of pvl gene which is present in highly virulent strains. In short, even though the study demonstrated the presence of MRSA at a lower rate, the results are of a marked public health significance due to the zoonotic significance of the pathogen.
Keywords: Mastitis, caprine, MRSA, PCR
