RESEARCH ARTICLES
Histological and ultrastructural characterisation as minimal criteria for assessing the success of the decellularisation protocols for tissue engineering applications
K. G. Megha, Dhanush Krishna Balakrishnan Nair, C. Divya, I. S. Sajitha, V. N. Vasudevan, P. R. Umashankar and S.S. Devi
doi: https://doi.org/10.51966/jvas.2022.53.2.208-213
Journal of Veterinary and Animal Sciences.2022.53(2):208-213
Author Details
K. G. Megha: MVSc Scholar,Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University, Kerala, India
Dhanush Krishna Balakrishnan Nair: Assistant Professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University, Kerala, India
C. Divya: Assistant Professor, Assistant Professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University, Kerala, India
I. S. Sajitha: Assistant Professor, Assistant Professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University, Kerala, India
V. N. Vasudevan: Assistant Professor, Department of Livestock Products Technology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University, Kerala, India
P. R. Umashankar: Scientist 'G' & Scientist-in-Charge, Division of In Vivo Models and Testing, Biomedical Technology Wing, Sree Chitra Thirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala-695 012
S.S. Devi: Assistant Professor, Assistant Professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University, Kerala, India
Article History
Received:14.07.2021 Accepted:15.09.2021,Published:30.06.2022
Corresponding author :Dhanush Krishna Balakrishnan Nair
email: dhanush@kvasu.ac.in
Citation: : Megha, K.G., Balakrishnan Nair, D.K., Divya, C., Sajitha, I.S., Vasudevan, V.N., Umashankar, P.R. and Devi, S.S. 2022. Histological and ultrastructural characterisation as minimal criteria for assessing the success of the decellularisation protocols for tissue engineering applications. J. Vet. Anim. Sci. 53(2): 208-213 DOI: https://doi.org/10.51966/jvas.2022.53.2.208-213
Abstract
There is a great demand of suitable biomaterials for tissue engineering applications in regenerative medicine. Decellularized extracellular matrix scaffolds derived from different organs and tissues have been successfully applied for human use as therapeutic agents in many tissue defects. The aim of the present study was to establish histological and ultrastructural characterisation as minimal criteria for assessing the suitability of decellularised bioscaffolds in tissue engineering applications. The study was performed on bovine omentum processed by a natural bovine bile based decellularisation protocol. Histological analysis and scanning electron microscopy examinations were performed and compared with an established decellularised bovine pericardial scaffold. Gross examination revealed that the decellularisation process did not alter the shape of the omentum. Histological examination confirmed the preservation of the collagenous fibres without any observable cell nuclei following decellularisation. Further, scanning electron microscopy examination demonstrated the arrangement and ultrastructure of collagen fibrils in the bioscaffolds. Our results suggest that histological and ultrastructural characterisation provide preliminary data on determining the biocompatibility of scaffolds before planning in vivo studies in tissue engineering applications.
Keywords: Biomaterial, bovine omentum, decellularisation
