RESEARCH ARTICLES
Antibiofilm activity of berberine and capsaicin in combination with quinolones against Staphylococcus aureus from bovine mastitis
Arya Mohan, A. R. Nisha, S. Sujith, S. Suja Rani and Naicy Thomas
doi: https://doi.org/10.51966/jvas.2022.53.2.253-261
Journal of Veterinary and Animal Sciences.2022.53(2):253-261
Author Details
Arya Mohan: MVSc Scholar, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
A. R. Nisha: Assistant Professor and Head, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
S. Sujith: Assistant Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
S. Suja Rani: Assistant Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
Naicy Thomas: Assistant Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
Article History
Received: : 05.11.2021 Accepted: 09.12.2021 Published:30.06.2022
Corresponding author :Arya Mohan
email : draryavet@gmail.com
Citation: Arya, M., Nisha, A. R., Sujith, S., Suja Rani, S. and Naicy, T. 2022. Antibiofilm activity of berberine and capsaicin in combination with quinolones against Staphylococcus aureus from bovine mastitis. J. Vet. Anim.Sci.53(2):253-261 DOI: https://doi.org/10.51966/jvas.2022.53.2.253-261
Abstract
Antimicrobial resistance which causes failure of antibiotic therapy has become a serious global issue nowadays. Bacteria develop resistance towards an antibacterial agent via many mechanisms. Biofilm formation by bacteria poses severe threat to the treatment of infections. The combination of plant molecules with antibiotics can combat antimicrobial resistance. In the present study, antibiofilm activity of two plant molecules berberine and capsaicin was investigated against Staphylococcus aureus isolates from bovine mastitis cases. Antibiotic sensitivity testing revealed that berberine and capsaicin when combined with quinolones increased the diameter of zone of inhibition in a dose dependent manner. In vitro biofilm assay using congo red agar plate method revealed the antibiofilm activity of berberine and capsaicin in combination with quinolone antibiotics. The study concludes that the combination of phytoconstituents with antibiotics can alleviate resistance mechanisms in bacteria. Keywords: Antimicrobial resistance, biofilm, Staphylococcus aureus, bovine mastitis
