RESEARCH ARTICLES
Molecular characterisation of virulence genes in Staphylococcus aureus associated with clinical bovine mastitis
Anugraha Mercy Easaw, K. Vijayakumar, K. Justin Davis, V.H. Shyma and S. Surya
doi: https://doi.org/10.51966/jvas.2022.53.2.279-284
Journal of Veterinary and Animal Sciences.2022.53(2):279-284
Author Details
Anugraha Mercy Easaw: MVSc Scholar,Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651 Kerala Veterinary and Animal Sciences University, Kerala, India
K. Vijayakumar: Professor and Head, , Department of Veterinary Epidemiology and Preventive Medicine,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651 Kerala Veterinary and Animal Sciences University, Kerala, India
K. Justin Davis: Assistant Professor,Department of Veterinary Epidemiology and Preventive Medicine,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651 Kerala Veterinary and Animal Sciences University, Kerala, India
V. H. Shyma: Assistant Professor,Department of Veterinary Epidemiology and Preventive Medicine,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651 Kerala Veterinary and Animal Sciences University, Kerala, India
S. Surya: Assistant Professor, Department of Veterinary Microbiology College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680651 Kerala Veterinary and Animal Sciences University, Kerala, India
Article History
Received:16.11.2021 ,Accepted: 14.12.2021,Published:30.06.2022
Corresponding author :Anugraha Mercy Easaw
email: mercyvet2013@gmail.com
Citation: Easaw, A.M., Vijayakumar, K., Davis, J., Shyma, V.H. and Surya, S.2022. Molecular characterisation of virulence genes in Staphylococcus aureus associated with clinical bovine mastitis. J. Vet. Anim. Sci. 53(2): 279-284 DOI: https://doi.org/10.51966/jvas.2022.53.2.279-284
Abstract
Staphylococcus aureus is the most frequently isolated pathogen from bovine mastitis including subclinical, clinical, and chronic infections. Virulence factors possessed by S. aureus aid in causing infection and inflammation by producing toxins and proteins, which are responsible for the pathogenesis of the disease. In the current study out of 51 animals presented with clinical mastitis, S. aureus was isolated from the milk of 18 animals. S. aureus was confirmed by genus and species level identification using polymerase chain reaction. Molecular characterization of selected virulence genes including thermonuclease (nuc) and Panton Valentine Leucocidin (PVL) was performed in all the S. aureus isolates. Presence of nuc gene was observed in all the isolates (100 %) of S. aureus. No isolates were found to be positive for the presence of PVL gene. Profiling the virulence genes is an important tool for epidemiological studies of mastitis, which can be employed for the prevention and control of the disease.
Keywords: Mastitis, Staphylococcus aureus, virulence genes
