RESEARCH ARTICLES

Association of Butyrophilin gene polymorphism (A465G) with milk production traits in Holstein Friesian crossbred cattle of Kerala
Potu Hemanth, F. A. Lali , K. Anilkumar, T.V. Aravindakshan, M. T. Dipu

doi:  https://doi.org/10.51966/jvas.2022.53.2.315-321

 Journal of Veterinary and Animal Sciences.2022.53(2):315-321

Author Details

Potu Hemanth: MVSc Scholar,Department of Animal Genetics and Breeding,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651,Kerala Veterinary and Animal Sciences University, Kerala, India.

F. A. Lali:  Assistant Professor ,Department of Animal Genetics and Breeding,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651,Kerala Veterinary and Animal Sciences University, Kerala, India.

K. Anilkumar: Professor,Department of Animal Genetics and Breeding,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651,Kerala Veterinary and Animal Sciences University, Kerala, India.

T.V. Aravindakshan:Professor and head,Department of Animal Genetics and Breeding,College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651,Kerala Veterinary and Animal Sciences University, Kerala, India.

M. T. Dipu: Assistant Professor, Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680 651 Kerala Veterinary and Animal Sciences University, Kerala, India

Article History

Received:08.12.2021,Accepted:21.12.2021,Published:30.06.2022 

Corresponding author :Potu Hemanth

email: hemanthpotu14@gmail.com

 

Citation:  Hemanth, P., Lali, F.A., Anilkumar, K., Aravindakshan, T.V. and Dipu, M.T. 2022. Associationof Butyrophilin gene polymorphism (A465G) with milk production traits in Holstein Friesiancrossbred cattle of Kerala. J. Vet.Anim.Sci.53(2):315-321

DOI:https://doi.org/10.51966/jvas.2022.53.2.315-321

 



Abstract


The butyrophilin (BTN1A1) gene is found at a quantitative trait locus (QTL) in cattle for milk production traits. The effect of a single nucleotide polymorphism (SNP) A465G in exon 8, which causes a lysine to arginine transition, on milk production attributes in Holstein Friesian crossbred cattle from Kerala was investigated in this study (151 numbers). Using custom synthesised primers, a 90-bp fragment encompassing the polymorphic region was amplified from genomic DNA isolated.Genotyping was carried out by high resolution melt curve analysis (HRM) and two genotypes KK (0.25) and KL (0.75) were detected based on melting temperature and melt curve patterns.Sanger’s sequencing and sequence analysis of representative samples confirmed the genotypes.Chi-square test showed that the population was not distributed as per Hardy-Weinberg equilibrium(p≤0.05). The relationship between the A465G transition and milk production traits like 305 daymilk, fat, SNF yields, fat and SNF per cent was determined by general linear model-analysis of variance (GLM-ANOVA). In the model herd, season of calving, parity of animal (non-geneticfactors), and genotype were considered fixed variables and milk production traits as dependent variable. The study revealed significantly higher (p≤0.01) milk fat and SNF per cent for KK genotype(4.14±0.08 %; 7.86±0.06 %) than KL genotype (3.91±0.07 %; 7.73±0.05 %). The butyrophilin genepolymorphism (A465G) can be recommended as a marker for higher milk fat and SNF per cent infuture breeding programmes in crossbred cattle of Kerala.

Keywords: Butyrophilin, SNP, high resolution melt curve, crossbred cattle, milk production traits