RESEARCH ARTICLES

In vitro antibacterial activity of andrographolide and hordenine against methicillin-resistant Staphylococcus aureus and Staphylococcus aureus
S. J. Vidyavarsha, Bibu John Kariyil, Varsha Unni, Jess Vergis, Sanis Juliet, Preethy John and R. Anoopraj

doi: : https://doi.org/10.51966/jvas.2022.53.3.407- 412

Journal of Veterinary and Animal Sciences.2022.53(3):407-412.

Author Details

S. J. Vidyavarsha: MVSc Scholar,Department of Veterinary Pharmacology and Toxicology, College of Veterinary and AnimalSciences, Pookode, 673576 Kerala Veterinary and Animal Sciences University, Kerala, India

Bibu John KariyilAssistant Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651. Kerala Veterinary and Animal Sciences University, Kerala, India.

Varsha Unni: PMVSc Scholar, Department of Veterinary Public healthCollege of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651. Kerala Veterinary and Animal Sciences University, Kerala, India

Jess Vergis: Assistant Professor, Department of Veterinary Public health, College of Veterinary and AnimalSciences, Pookode, 673576 Kerala Veterinary and Animal Sciences University, Kerala, India

Sanis JulietAssociate Professor and Head, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and AnimalSciences, Pookode, 673576 Kerala Veterinary and Animal Sciences University, Kerala, India

Preethy John: Assistant Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and AnimalSciences, Pookode, 673576 Kerala Veterinary and Animal Sciences University, Kerala, India

R. Anoopraj: Assistant Professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Pookode, 673576. Kerala Veterinary and Animal Sciences University, Kerala, India.

Article History

Received: 12.02.2022, Accepted: 31.03.2022, Published online: 30.09.2022

Corresponding authorBibu John Kariyil

e-mail: bibujohn@kvasu.ac.in

Citation: Vidyavarsha, S. J., Bibu John Kariyil, Varsha Unni, Jess Vergis, Sanis Juliet, Preethy Johnand Anoopraj R. 2022. J. Vet. Anim. Sci. 53 (3): 407-412

DOI: https://doi.org/10.51966/jvas.2022.53.3.407-412



Abstract


Bacterial infections are difficult to treat, especially those infected with resistant bacteria. Due to the haphazard use of antibiotics in humans and animals, methicillin-resistant Staphylococcus aureus (MRSA) has developed resistance to practically all commercially available antibacterial drugs. Exploring natural plant compounds with antibacterial activity could be beneficial in the treatment of infections with MRSA and Staphylococcus aureus. Andrographolide, a labdane diterpenoid derived from Andrographis paniculata, and Hordenine, a phenethylamine alkaloid found in Hordeum vulgare, are reported to have antibacterial activity. In the present study, the antibacterial activity of andrographolide and hordenine was tested against MRSA and S. aureus using the Kirby- Bauer agar disc diffusion method and the modified resazurin microtiter plate assay. The results of the study revealed that both andrographolide and hordenine at various concentrations did not show any zone of inhibition against MRSA and S. aureus. Hordenine showed an MIC of 1000 μg/mL against MRSA and S. aureus, but andrographolide had no effect on both MRSA and S. aureus.

Keywords: Andrographolide, Hordenine, antibacterial activity, MRSA, S. aureus