RESEARCH ARTICLES

Effect of Malabari goat lactoferrin on tumour growth kinetics in experimentally induced tumours in mice
C. G. Mukul, R. Uma, K. K. Jayavardhanan, Preethy John, B. Dhanush Krishna and S. V. Vasudhar Bhat

doi: : https://doi.org/10.51966/jvas.2022.53.3.424- 428

Journal of Veterinary and Animal Sciences.2022.53(3):424-428.

Author Details

C. G. Mukul: MVSc Scholar, Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University. Kerala, India.

R. Uma: Assistant Professor and Head (i/c), Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University. Kerala, India.

K. K. Jayavardhanan: Professor (Retired), Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University. Kerala, India.

Preethy John: Assistant Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University, Kerala, India

B. Dhanush Krishna: Assistant Professor, Department of Veterinary Pathology College of Veterinary and AnimalSciences, Mannuthy,680651 Kerala Veterinary and Animal Sciences University, Kerala, India

S. V. Vasudhar Bhat: PhD Scholar, Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651 Kerala Veterinary and Animal Sciences University. Kerala, India.

Article History

Received: 15.02.2022, Accepted: 21.03.2022, Published online: 30.09.2022

Corresponding author: R. Uma.

e-mail: uma@kvasu.ac.in

Citation: Mukul, C. G., Uma, R., Jayavardhanan. K. K., John, P., Dhanush Krishna, B and Vasudhar Bhat, S.V. 2022. Effect of Malabari goat lactoferrin on tumour growth kinetics in experimentally induced tumours in mice. J. Vet. Anim. Sci. 53 (3): 424-428

DOI: https://doi.org/10.51966/jvas.2022.53.3.424-428



Abstract


Lactoferrin, a minor whey protein, possesses a multitude of biological functions including antimicrobial, immunomodulatory and antitumour activities. The present study focuses on the antitumour activity of lactoferrin isolated from Malabari goats. Lactoferrin was isolated from Malabari goat colostrum by Cation exchange chromatography. The purity of the isolated protein was confirmed by SDS-PAGE and its identity was ascertained by Western Blotting. Solid tumours were induced in hind limbs of Swiss Albino mice using Daltons lymphoma ascites cells. Animals which developed the tumours were divided into groups and administered different doses of Malabari goat lactoferrin (MgLf) intratumourally as a single dose. The tumour positive animals were sacrificed on day 10 post treatment to study the effects of MgLf on tumour growth kinetics by assessing the tumour weight: body weight ratio and tumour volume inhibition of the slaughtered animals. It was found that MgLf @150μg significantly lowered the tumour weight: body weight ratio and tumour volume of the treated animals.

Keywords: Lactoferrin, Malabari goat, Daltons ascites lymphoma, tumour weight: body weight ratio.