RESEARCH ARTICLES
Identification and detection of virulence genes among Pseudomonasspp isolated from Wayanad
R. G. Sacharia, K. C. Bipin, P. M. Deepa, R. L. Rathish and C. Ravishankar
doi: https://doi.org/10.51966/jvas.2022.53.4.651-656
Journal of Veterinary and Animal Sciences.2022.53(4):651-656
Author Details
R. G. Sacharia: MVSc Scholar, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala
K. C. Bipin : Assistant Professor, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala.
P. M. Deepa : Assosiate Professor and Head i/c, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala.
R. L. Rathish : Assistant Professor, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala.
C. Ravishankar : Professor, Dept. of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala Veterinary and Animal Sciences University, Kerala, India.
Article History
Received: 18.06.2022, Accepted: 19.09.2022, Published online: 31.12.2022
Corresponding author: K. C. Bipin
e-mail : kcbipin@gmail.com
Citation: Sacharia,R.G., Bipin, K.C., Deepa, P.M., Rathish, R.L. and Ravishankar, C. 2022. Identification and detection of virulence genes among Pseudomonas spp isolated from Wayanad. J. Vet. Anim. Sci. 53(4): 651-656
DOI: https://doi.org/10.51966/jvas.2022.53.4.651-656
Abstract
study was conducted to identify virulence genes of Pseudomonas spp. from 20 cases of canine otitis externa in Wayanad district of Kerala. The samples were collected aseptically and bacterial isolates were identified using culture and biochemistry. The nine identified Pseudomonas isolates were selected and further confirmed by PCR, targeting Opr I gene at genus level. Presence of virulence genes viz. Las A, Las B, Lec A, Lec B and Apr were examined in the confirmed isolates. All the samples were positive for Las B and Apr. Five among the isolates were having Lec A gene. None of the isolates possessed Lec B and Las A gene. Presence of Las B and Apr gene indicate biofilm forming ability of the isolated Pseudomonas organisms.
Keywords: Canine otitis externa, Pseudomonas, PCR, virulence genes
