RESEARCH ARTICLES
Molecular detection of Mycobacterium tuberculosis complex in bovine blood samples
N. Sarika, B. K. Mani, M. Mini, P. M. Priya, B. Sunil, Ajith Jacob George and K. L. Sarada Devi
doi: https://doi.org/10.51966/jvas.2022.53.4.699-702
Journal of Veterinary and Animal Sciences.2022.53(4):699-702.
Author Details
N. Sarika: Ph. D Scholar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India.
B.K.Mani: Assosiate Professor,Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur -680651, Kerala Veterinary and Animal Sciences University, Kerala, India.
M. Mini : Senior Professor and Head, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy - 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India.
P. M. Priya: Professor , Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy - 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India.
B. Sunil : Professor and Head, Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy - 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India.
Ajith Jacob George : Professor and Head, Department of veterinary Pathology, College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala Veterinary and Animal Sciences University, Kerala, India
K. L. Sarada Devi : Professor and Head (Retd.), Department of Microbiology, Government Medical College,Thiruvananthapuram
Article History
Received: 28.06.2022, Accepted: 12.09.2022, Published online: 31.12.2022
Corresponding author: B. K. Mani
e-mail : binu@kvasu.ac.in
Citation: Sarika, N., Mani, B.K., Mini, M., Priya, P.M., Sunil, B., George, A.J. and Sarala Devi, K.L. 2022. Molecular detection of Mycobacterium tuberculosis complex in bovine blood samples. J. Vet. Anim. Sci. 53(4): 699-702
DOI: https://doi.org/10.51966/jvas.2022.53.4.699-702
Abstract
Bovine tuberculosis (bTB), predominantly caused by Mycobacterium bovis, is one of the most neglected zoonotic diseases of cattle. The lack of documented information on bTB is one of the most important hurdles in controlling the disease. The present study was carried out to detect the presence of Mycobacterium tuberculosis complex (MTBC) from blood samples of cattle presented for slaughter, using polymerase chain reaction. The study revealed presence of MTBC in seven out of 50 animals (fourteen per cent) with symptoms suggestive of bTB. The detection of the organism emphasises the need of further prevalence studies in Kerala, to implement proper control strategies.
Keywords: Bovine tuberculosis, MTBC, IS6110 PCR
