RESEARCH ARTICLES

Comparison of California Mastitis Test and somatic cell counts for detection of subclinical mastitis in crossbred cattle
Arifa Abdulkhader, K.V. Meganath, S. Vignesh, P.M. Rojan, E.M. Muhammed, R.L. Rathish, V.N. Muhasin Asaf and C.N. Dinesh

doi: https://doi.org/10.51966/jvas.2022.53.4.725-730

Journal of Veterinary and Animal Sciences.2022.53(4):725-730.

Author Details

Arifa Abdulkhader : MVSc Scholar, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode, Wayanad-673576, Kerala Veterinary and Animal Sciences University, Kerala, India

K.V. Meganath : MVSc Scholar, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode, Wayanad-673576, Kerala Veterinary and Animal Sciences University, Kerala, India

S. Vignesh: MVSc Scholar, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode, Wayanad-673576, Kerala Veterinary and Animal Sciences University, Kerala, India

P.M. Rojan: Assistant Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode, Wayanad-673576, Kerala Veterinary and Animal Sciences University, Kerala, India

E.M. Muhammed :  Assistant Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode, Wayanad-673576, Kerala Veterinary and Animal Sciences University, Kerala, India

R.L. Rathish : Assistant Professor, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Pookode, Wayanad-673576, Kerala Veterinary and Animal Sciences University, Kerala, India

V.N. Muhasin Asaf : Assistant Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Pookode, Wayanad-673576, Kerala Veterinary and Animal Sciences University, Kerala, India

C.N. Dinesh : Professor, Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India

Article History

Received: 29.06.2022, Accepted: 03.09.2022, Published online: 31.12.2022

Corresponding author: Arifa Abdulkhader

e-mail : arifakhader11@gmail.com

Citation:  Arifa, A., Meganath, K.V., Vignesh, S., Rojan, P.M., Muhammed, E.M., Rathish, R.L., Muhasim Asaf, V. N. and Dinesh, C.N. 2022 Comparison of California Mastitis Test and somatic cell counts for detection of subclinical mastitis in crossbred cattle. J. Vet. Anim. Sci. 53(4): 725-730

DOI: https://doi.org/10.51966/jvas.2022.53.4.725-730



Abstract


Mastitis is a major constraint that severely affects milk production in dairy animals. The California Mastitis Test (CMT) is a reliable and rapid field test for the diagnosis of subclinical mastitis (SCM) which gives an indirect estimate of somatic cell count (SCC). Based on the results of CMT screening and SCC of the milk of 105 crossbred animals located in different farms in Wayanad and Calicut districts of Kerala state, the present study attempts to find the estimates of sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV), false discovery rate (FDR) and false omission rate (FOR) of CMT relative to SCC as the reference standard. The correlation and agreement between CMT and SCC were also analysed. The estimated Spearman and Kendall Tau b correlation coefficients were 0.88 and 0.76, respectively, which indicated a strong positive relationship between CMT and SCC. The sensitivity and specificity values of CMT were 1.000±0.000 and 0.510±0.071, respectively. These values indicate that the probability for an animal with mastitis to be identified using CMT is 100 per cent and the probability of correctly identifying an animal without mastitis animal is 51 per cent. The high sensitivity value of CMT in this study indicated that CMT could be used to find out the true prevalence of SCM in crossbred animals. Analysis of the data also revealed that CMT had a PPV of 0.700±0.051 and an NPV of 1.000±0.000. The calculated accuracy of CMT was 0.771±0.041. The estimated FDR and FOR were 0.300±0.051 and 0.000±0.000, respectively. Kappa statistic was used to determine the level of agreement between CMT and SCC and the kappa coefficient value was 0.53±0.07 which indicated moderate agreement.

Keywords: Subclinical mastitis, California Mastitis Test, somatic cell count, correlation