RESEARCH ARTICLES

Polymorphism study of TLR4 gene in crossbred cattle of Kerala
G. Chishi Kiyevi, Valsalan Jamuna, Sadan Tina, K. Anilkumar, T. V. Aravindakshan and K. Radha

doi: https://doi.org/10.51966/jvas.2023.54.1.1-6

Journal of Veterinary and Animal Sciences.2023.54(1):1-6

Author Details

G. Chishi Kiyevi : MVSc Scholar, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur.

Valsalan Jamuna : Assistant Professor, Centre for Advanced Studies in Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur.

Sadan Tina : Junior Research Fellow, DST-SERB, Centre for Advanced Studies in Animal Genetics and Breeding

K. Anilkumar : Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy,  Thrissur

T. V. Aravindakshan : Professor and Head, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur.

K. Radha : Assistant Professor, Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, Thrissur.

Article History

Received: 03.08.2021 Accepted: 13.09.2021 Published online: 31.03.2023

Corresponding author: G. Chishi Kiyevi

e-mail : kevinchishi86@gmail.com

Citation: Kiyevi,G.C., Jamuna,V., Tina,S., Anilkumar,K., Aravindakshan,T.V. and Radha,K. 2023. Polymorphism study of TLR4 gene in crossbred cattle of Kerala. J. Vet. Anim. Sci. 54(1):1-6

DOI: https://doi.org/10.51966/jvas.2023.54.1.1-6

 

 



Abstract


The present study was conducted to identify single nucleotide variations in exon 3 region of the bovine Toll-Like Receptor gene (TLR4) associated with somatic cell score (SCS) in crossbred cattle of Kerala using polymerase chain reaction-single stranded conformational polymorphisms (PCR-SSCP) technique. Blood samples were collected from 200 lactating crossbred cattle in different farms of Kerala Veterinary and Animal Sciences University (KVASU) and field centres of ICAR- FPT scheme, KVASU. Genomic DNA was isolated and polymorphisms were identified by SSCP analysis. The 231 bp fragment of exon 3 in TLR4 gene was found to polymorphic and two genotypes viz. CC and CD were obtained with frequencies 77 per cent and 23 per cent respectively. The frequencies of C and D allele in crossbred were found to be 0.88 and 0.12. On sequencing, C to T transition had led to one non-synonymous change at 2021th position of ORF. Cattle with CC genotype were associated with significantly lower SCS (p≤0.05) as compared to CD genotype. The association between TLR4 polymorphism and SCS described in this study suggests the possible role of SNP during mastitis infection. Thus, could be employed as a useful marker for genetic selection to promote mastitis resistance in dairy cattle.

Keywords: TLR4, PCR-SSCP, somatic cell score, crossbred cattle