RESEARCH ARTICLES

Dimethylacetamide - an alternative to glycerol as cryoprotectant of Malabari buck semen
T. M. Kanimozhi, Leeba Chacko, K. Promod, and Abdul Azeez

doi: https://doi.org/10.51966/jvas.2023.54.1.64-70

Journal of Veterinary and Animal Sciences.2023.54(1):64-70

Author Details

T. M. KanimozhiMVSc. scholar, Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Pookode, Wayanad - 673576,  Kerala Veterinary and Animal Sciences University

Leeba ChackoAssistant Professor, Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Pookode, Wayanad - 673576,  Kerala Veterinary and Animal Sciences University

K. PromodProfessor and Head (i/c), Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Pookode, Wayanad - 673576,  Kerala Veterinary and Animal Sciences University

Abdul Azeez:  Assistant Professor, Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Pookode, Wayanad - 673576,  Kerala Veterinary and Animal Sciences University

Article History

Received: 02.08.2022 Accepted: 17.12.2022 Published online: 31.03.2023

Corresponding author: T. M. Kanimozhi

e-mail : drkanivet@gmail.com

Citation Kanimozhi,T.M., Chacko,L., Pramod,K. and Azeez,A. 2023. Dimethylacetamide - an alternative to glycerol as cryoprotectant of Malabari buck semen. J. Vet. Anim. Sci. 54(1):64-70

DOI: https://doi.org/10.51966/jvas.2023.54.1.64-70



Abstract


The key role of a cryoprotectant is to minimize the chemical and physical stress which occurs during cooling, freezing and thawing of semen. The difference between the cryoprotectant (CPA) occurs in their permeability coefficient and the structural model of the cryogenic agent. The beneficial effect of dimethylacetamide (DMA) as a cryoprotectant especially for sperms had been observed in several studies. The aim of the study was to study the cryoprotective effect of DMA in freezing the Malabari buck semen compared to glycerol. Ten ejaculates were taken from fourMalabaribucks . After preliminary evaluation sample split technique was followed with Tris based extender containing glycerol (6.7 per cent) as cryoprotectant (control) and Trisextender containing DMA (3 per cent) as cryoprotectant (treatment group). The semen straws (0.25mL) after filling were subjected for equilibration and manual freezing. Sperm kinetics was studied using computer-aided sperm analyzer. Pre-freeze and post-thaw evaluation included sperm viability, sperm abnormality, hypo osmotic test, acrosome integrity test and DNA fragmentation. Results indicated that inclusion of 6.7 per cent glycerol had significantly higher (p<0.05) post-thaw values than DMA. From our study we conclude that 6.7 per cent glycerol was better than 3 per cent DMA in cryopreservation of Malabari buck semen.

Keywords: Cryopreservation, dimethylacetamide, glycerol