RESEARCH ARTICLES

Evaluation of keeping quality of canine platelet rich plasma under different storage conditions
Deny Jennes, Soumya Ramankutty, S. Anoop, N. Madhavan Unny, S. Sudheesh Nair and K.D. John Martin

doi: https://doi.org/10.51966/jvas.2023.54.1.91-97

Journal of Veterinary and Animal Sciences.2023.54(1):91-97

Author Details

Deny Jennes: MVSc. scholar, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651, Kerala Veterinary and Animal Sciences University, Pookode, Kerala, India.

Soumya Ramankutty: Assistant Professor, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651, Kerala Veterinary and Animal Sciences University, Pookode, Kerala, India.

S. Anoop : Professor and Head, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala Veterinary and Animal Sciences University, Pookode, Kerala, India.

N. Madhavan Unny:  Professor and Head, Department of Clinical Medicine, Ethics and Jurisprudence, College of Veterinary and Animal Sciences, Pookode,  Kerala Veterinary and Animal Sciences University, Kerala, India.

S. Sudheesh Nair : Assistant Professor, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651, Kerala Veterinary and Animal Sciences University, Pookode, Kerala, India.

K.D. John Martin : Professor and Head, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur-680651, Kerala Veterinary and Animal Sciences University, Pookode, Kerala, India.

Article History

Received: 14.09.2022 Accepted: 01.11.2022 Published online: 31.03.2023

Corresponding author: Deny Jennes

e-mail : denyjennis@gmail.com

Citation : Jennes,D., Ramankutty,S., Anoop,S., Madhavan Unny,N., Sudheesh,S.N. and Martin, J.K.D. 2023. Evaluation of keeping quality of canine platelet rich plasma under different storage conditions. J. Vet. Anim. Sci. 54(1):91-97

DOI: https://doi.org/10.51966/jvas.2023.54.1.91-97



Abstract


Platelet rich plasma (PRP) therapy is an integral part of regenerative medicine as the platelets possess a good healing capacity owing to the presence of a wide variety of growth factors in the platelet granules found in the cytoplasm of the platelet. Autologous PRP was prepared from the blood of the patient itself, without any preservatives. Storage of PRP was one of the main hurdles of the treatment modality. During storage, the platelet counts may get reduced, undergo activation or get contaminated with bacteria as no preservatives are used in the preparation of autologous PRP. Cytological changes and microbial quality of the PRP during storage at 4oC and -20oC for seven days were analysed in this study. Reduction in platelet count and the chance of microbial contamination were less when autologous PRP was stored at -20°C compared to 4°C.

Keywords: Canine, autologous platelet rich plasma, PRP, double centrifugation method, storage changes