Qualitative and quantitative phytochemical analysis of methanolic extract of Magnolia champaca leaves
R. Shankar,Preethy John, A. R. Nisha, S. Sujith, R. Uma, V. Keerthika and Archana Raj
Journal of Veterinary and Animal Sciences.2023.54(1):204-213
Received: 08.11.2022 Accepted: 21.12.2022 Published online: 31.03.2023
Plants remain a vibrant source of diverse bioactive phytochemicals that are secondary metabolites protecting them from infections and predations. Magnolia champaca is reported to possess a multitude of phytochemicals. In the present study, the phytochemical constituents of the methanolic extract of Magnolia champaca leaves were analysed qualitatively and with gas chromatography-tandem mass spectrometry (GC-MS/MS). Fourier transform infrared (FTIR) spectroscopic analysis was performed to identify the chemical nature of the extract and to find structurally similar compounds. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, terpenes, glycosides, phenols, steroids, tannins and saponins. FTIR spectroscopic analysis revealed structurally related compounds. GC-MS/MS analysis revealed the presence of 99 diverse compounds with varied biological activities, among which 1,2,4-butanetriol, n-hexadecanoic acid, cis vaccenic acid, phytol, trans longipinocarveol and caryophyllene oxide were found predominantly. Thus, the identification and characterisation of the phytochemicals in the extract favour the development of novel therapeutic agents.
Keywords: Magnolia champaca, GC-MS/MS, FTIR, phytochemicals, phytol, caryophyllene oxide