RESEARCH ARTICLES
Expression study of pro-inflammatory cytokines and acute phase proteins in post-partum crossbred dairy cows with subclinical endometritis
Sowmya Murali, Leeba Chacko, Renjith Sebastian, K. Promod and C. P. Abdul Azeez
doi: https://doi.org/10.51966/jvas.2023.54.2.450-456
Journal of Veterinary and Animal Sciences.2023.54(2):450-456
Author Details
Sowmya Murali : MVSc Scholar, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Pookode, Wayanad -673576, Kerala Veterinary and Animal Sciences University, Kerala, India
Leeba Chacko : Assistant Professor, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Pookode, Wayanad -673576, Kerala Veterinary and Animal Sciences University, Kerala, India
Renjith Sebastian : Assistant Professor, Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Pookode, Wayanad -673576,Kerala Veterinary and Animal Sciences University, Kerala, India
K. Promod : Professor and Head, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Pookode, Wayanad -673576, Kerala Veterinary and Animal Sciences University, Kerala, India
C. P. Abdul Azeez : Associate Professor, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Pookode, Wayanad -673576, Kerala Veterinary and Animal Sciences University, Kerala, India
Article History
Received: 11.11.2022 Accepted: 02.02.2023 Published online: 30.06.2023
Corresponding author: Leeba Chacko
e-mail : leeba@kvasu.ac.in,
Citation: Murali, S., Chacko, L., Sebastian, R., Promod, K. and Azeez, C. P. A. 2023. Expression study of pro-inflammatory cytokines and acute phase proteins in post-partum crossbred dairy cows with subclinical endometritis. J. Vet. Anim. Sci. 54(2):450-456
DOI: https://doi.org/10.51966/jvas.2023.54.2.450-456
Abstract
Subclinical endometritis (SCE) is one of the causes of poor reproductive performance in dairy cows. The objective of the study was to evaluate the expression of pro-inflammatory cytokines [PICs; Interleukin -1 beta (IL-1β), Interleukin -6 (IL-6), and Tumor necrosis factor- alpha (TNF-α)] and Acute phase proteins [APPs; Serum Amyloid- A (SAA) and Haptoglobin (HP)] in blood and uterine flushings of postpartum dairy cows with subclinical endometritis (SCE). Animals (n=49) were screened for SCE at 60 days postpartum (dpp) using the endometrial cytobrush cytology, and those with PMN cell percentage ≥ 18% were identified as SCE cows. The blood samples and uterine flushings from SCE (n=15) and the control group (n=15) were assessed for mRNA expressions of PICs and APPs using the Real-Time PCR method (RT-PCR). The PICs and APPs except TNF-α were non-significant (p>0.05) in blood samples whereas a significant difference (p<0.05) was found in uterine flushings. The PICs and APPs were significantly up-regulated in the uterine endometrium indicating that collection of uterine flushings at 60 dpp could be used as a diagnostic method for evaluating gene expressions of inflammatory mediators in dairy cows with SCE.
Keywords: Endometrial cytobrush, pro-inflammatory cytokines, acute phase proteins
