RESEARCH ARTICLES

N gene based detection and phylogenetic analysis of canine morbillivirus in dogs
K. M. Maneesh, K. Sumod, Chintu Ravishankar, Koshy John, R. Rajasekhar, P. M. Arun, S. Shashank, G. S. Anjitha, P. Jishnu Haridas, K. V. Jayanth, N. Madhan Raj and L. Sri Ramya

doi: https://doi.org/10.51966/jvas.2023.54.2.486-491

Journal of Veterinary and Animal Sciences.2023.54(2):486-491

Author Details

K. M. Maneesh : MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

K. Sumod : Assistant Professor, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

Chintu Ravishankar : Professor, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

Koshy John : Professor and Head, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

R. Rajasekhar : Assistant Professor, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India 

P. M. Arun : MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

S. Shashank : MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

G. S. Anjitha : MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

 P. Jishnu Haridas : MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

K. V. Jayanth : MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

N. Madhan Raj : MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

L. Sri Ramya : MVSc Scholar, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Pookode, Wayanad-673 576, Kerala Veterinary and Animal Sciences University, Kerala, India

 Article History

Received: 14.11.2022 Accepted: 06.01.2023 Published online: 30.06.2023

Corresponding author: K. M. Maneesh

e-mail : drmaneeshmohan95@gmail.com

CitationManeesh, K.M., Sumod, K., Ravishankar, C., John, K., Rajasekhar, R., Arun, P.M., Shashank, S., Anjitha, G.S., Haridas, J.P., Jayanth, K.V., Raj. M.N. and Ramya, S.L. 2023. N gene based detection and phylogenetic analysis of canine morbillivirus in dogs. J. Vet. Anim. Sci. 54(2):486-491

DOI: https://doi.org/10.51966/jvas.2023.54.2.486-491



Abstract


Canine distemper, a highly fatal systemic disease in domestic dogs and wild carnivores, has the second highest mortality rate after rabies and is responsible for a large number of animal deaths around the world. It is considered a major pathogen in the canine infectious respiratory disease complex. This paper reports the finding of a study conducted to detect and characterise canine distemper virus (Canine Morbillivirus) based on N gene. A total of 59 samples collected from cases of respiratory infections in dogs were subjected to N gene based reverse transcriptase polymerase chain reaction (RT-PCR) and eleven of them (18.64 per cent) were found positive. Sequencing and phylogenetic analysis revealed that all the canine distemper viruses obtained in the present study were related to Indian strains that were previously reported. However, the viruses from the same district were similar among themselves.

Keywords: Canine distemper virus, phylogenetic analysis, reverse transcriptase-polymerase chain reaction, N gene