RESEARCH ARTICLES
Adult immersion test for assessment of amitraz resistance in Rhipicephalus sanguineus ticks
Shaik Nikhat Reena, Bindu Lakshmanan, N. Suresh Nair K. Syamala, R. Radhika, P. K. Krishnanunni and S. Devi Chandana
doi: https://doi.org/10.51966/jvas.2023.54.2.532-536
Journal of Veterinary and Animal Sciences.2023.54(2):532-536
Author Details
Shaik Nikhat Reena : MVSc Scholar, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences,Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
Bindu Lakshmanan : Professor and Head, Department of Parasitology, College of Veterinary and Animal Sciences,Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
N. Suresh Nair : Associate Professor, Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences,Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
K. Syamala : Assistant Professor, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences,Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
R. Radhika : Associate Professor , Department of Veterinary Parasitology, College of Veterinary and Animal Sciences,Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
P. K. Krishnanunni : Graduate scholar, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences,Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
S. Devi Chandana : Graduate scholar, Department of Veterinary Parasitology, College of Veterinary and Animal Sciences,Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
Article History
Received: 15.11.2022 Accepted: 09.01.2023 Published online: 30.06.2023
Corresponding author: Shaik Nikhat Reena
e-mail : nikhat.reena@gmail.com
Citation: Reena, S.N., Lakshmanan, B., Nair, S.N., Syamala, K., Radhika, R., Krishnanunni, P.K. and Chandana, S.D. 2023. Adult immersion test for assessment of amitraz resistance in Rhipicephalus sanguineus ticks. J. Vet. Anim. Sci. 54(2):532-536
DOI: https://doi.org/10.51966/jvas.2023.54.2.532-536
Abstract
Ticks are obligate blood sucking ectoparasites recognised globally as vectors of various pathogens, next to mosquitoes. The present study is conducted on brown dog ticks collected from naturally infested dogs in and around Thrissur district. Adult immersion test was performed and the parameters such as engorged tick weight, egg mass weight as well as tick mortality were recorded to derive the reproductive indices, percentage inhibition of oviposition and percentage mortalities. Non-linear regression analysis of the dose response data yielded the LC50 values at 95% confidence intervals and, the slopes and regression coefficients of the different parameters were also derived. The Resistance factors (RF) were calculated to categorise resistance levels into I, II, III and IV. Resistance to amitraz was detected at level II in tick isolates collected from Choolissery and Mundur, at level III and level IV in isolates collected from Mannuthy and Paravattani, respectively. Two isolates collected from Mannuthy were found to be susceptible. One way ANOVA of resistance levels revealed that the resistance levels III and IV were significantly different from that of susceptible and resistance level II. This is the first report on detection of amitraz resistance levels in dog ticks from south India calling for proper implementation of tick control strategies to prevent spread of amitraz resistance.
Keywords: Adult immersion test, brown dog ticks, resistance levels, Kerala
