RESEARCH ARTICLES
Diagnosis of canine brucellosis using Rose Bengal plate test
K. Athira, V.H. Shyma, K.D. Justin, K. Vijayakumar, C. Jayakumar and J. Greeshma
doi: https://doi.org/10.51966/jvas.2023.54.2.544-551
Journal of Veterinary and Animal Sciences.2023.54(2):544-551
Author Details
K. Athira : PhD Scholar, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy- 680 651, Thrissur, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, India
V.H. Shyma : Assistant Professor, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy – 680651, Kerala Veterinary and Animal Sciences University, Kerala,India
K.D. Justin : Assistant Professor, Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy – 680651, Kerala Veterinary and Animal Sciences University, Kerala,India
K. Vijayakumar : Dean, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
C. Jayakumar : Associate Professor and Head, Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
J. Greeshma : Teaching Assistant, Department of Statistics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
Article History
Received: 03.05.2023 Accepted: 22.06.2023 Published online: 30.06.2023
Corresponding author: K. Athira
e-mail :athirakadankandath1993@gmail.com
Citation: Athira, K., Shyma, V. H., Justin, K. D., Vijayakumar, K., Jayakumar, C. and Greeshma, J. 2023. Diagnosis of canine brucellosis using Rose Bengal plate test. J. Vet. Anim. Sci. 54(2):544- 551
DOI: https://doi.org/10.51966/jvas.2023.54.2.544-551
Abstract
Canine brucellosis or beagle fever is a zoonotic bacterial reproductive disease of dogs, caused by Brucella canis and occasionally by Brucella abortus, B. melitensis and B. suis. The actual seroprevalence of canine brucellosis in India is unknown and not yet studied in Kerala. A total of 131 animals presented to the outpatient unit of medicine, gynaecology and obstetrics of the two University Veterinary Hospitals at Mannuthy and Kokkala with clinical signs of epididymitis, orchitis, abortion, still birth, foetal resorption, foetal mummification, foetal maceration, neonatal death and infertility were randomly selected for the study. Paired sera samples were collected on the day of presentation and after three weeks of presentation for Rose Bengal Plate Test (RBPT). In this study, sera samples from forty-seven (35.88 per cent) infected dogs showed agglutination on RBPT using B. abortus S99 antigen. Out of forty-seven RBPT positive samples, 80.85 per cent (38/47) were female and 19.15 per cent (9/47) were male dogs. The high seroprevalence of canine brucellosis in this study is attributed to the endemicity of bovine brucellosis in the study area.
Keywords: Canine brucellosis, epididymitis, orchitis, abortion, neonatal death, infertility, Rose
Bengal Plate Test (RBPT)
