RESEARCH ARTICLES
Genetic variability at exon 2 of inhibin alpha locus in malabari goats of Kerala
Haritha B. Pillai, R. Thirupathy Venkatachalapathy, T.V. Aravindakshan, Jamuna Valsalan, K. Raji
Journal of Veterinary and Animal Sciences.2020. 51(1):82-85.
Author Details
Haritha B. Pillai: PG Scholar, School of Applied Animal Production and Biotechnology, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
R. Thirupathy Venkatachalapathy: Professor and Head, Livestock Research Station, Thiruvazhamkunnu, Kerala Veterinary and Animal Sciences University, Kerala, India.
T. V. Aravindakshan: Director, Centre for Advanced studies in Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
Jamuna Valsalan: Assistant Professor, Centre for Advanced studies in Animal Genetics and Breeding,College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
K. Raji: Associate Professor, Department of Veterinary Physiology, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Kerala, India.
Article History
Received: 06.08.2019, Accepted: 18.09.2019, Published online: 01.01.2020
Corrersponding author: Haritha B. Pillai
e-mail: bpillai.haritha@gmail.com
Citation: Pillai, H. B., Venkatachalapathy, R. T., Aravindakshan, T.V., Valsalan, J. and Raji K. 2020. Genetic variability at exon 2 of inhibin alpha locus in Malabari goats of Kerala. J. Vet. Anim. Sci. 51(1): 82-85.
Abstract
The present study included 207 Malabari goats from six centers viz., University Goat and Sheep Farm, Mannuthy and 5 field centres of the ICAR-All India Coordinated Research Project on Goat Improvement (Malabari). The study was planned to detect single nucleotide variations in inhibin alpha gene of Malabari goats. Genomic DNA was isolated from 207 animals and PCR was done to amplify 220 bp fragment of exon 2 region of inhibin alpha (INHA) gene. Single strand conformation polymorphism (SSCP) technique was used to detect single nucleotide polymorphisms (SNPs). The SSCP showed a similar pattern of two bands (CC) for all samples and the population was found to be monomorphic for the locus.
Key words:Malabari goat, inhibin alpha, exon, SSCP
