4. PREPARATION OF EXCRETORY SECRETORY PROTEIN FROM BRUGIAN MICROFILARIAE ISOLATED FROM CANINE BLOOD
Poojary Vineeta Sadarama, Deepa Chirayath,Usha Narayana Pillai, Madhavan Unny and Bindu Lakshmanan
The article describes the culture of microfilaria of Brugia spp. for the extraction of excretory-secretory protein. The microfilariae were separated from the various components of canine blood using HiSep™ LSM by gradient centrifugation method. The microfilariae were cultured in RPMI-1640 media and incubated at 37°C. The spent media was collected at 12 hour intervals, filtered, pooled and stored at -50°C. Protein estimation was done by Bradford’s method. The excretory-secretory protein produced can further be used in diagnostic tests.
Keywords: Excretory secretory protein, Brugiasp. HiSep, Canine.