3. FREEZE DRYING AND RECOVERY OF Aspergillus flavus NRRL 6513 STRAIN
A.Aneesh, A. J. George and M.V.Chinnu
Journal of Veterinary and Animal Sciences.2019. 50(1):74-76.
Copyright: © 2019 Aneesh et.al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Study was undertaken to assess the effectiveness of lyophilisation in the preservation and recovery of Aspergillus flavus NRRL 6513 strain. The stock culture with known level of Aflatoxin B1 production ability was periodically subcultured in potato dextrose agar at 15 days interval to maintain its viability. Skim milk solution was used for the preservation of stock culture. The fungal culture was lyophilised and vacuum packed. The lyophilised fungus was recovered and aflatoxins B1 level was checked again in maize culture. The post lyophilisation level of Aflatoxin B1 was comparable to the amount produced by stock culture.
Keywords : Aspergillus flavus, NRRL 6513 STRAIN, Freeze drying