Comparing ELISA using serum antibodies and coproantigens for detecting early amphistomosis
A Kandasamy and K Devada
Department of Veterinary Parasitology College of Veterinary and Animal Scineces Mannuthy 680 651, Thrissur, Kerala, India
Hundred cattle known to be infected with Gastrothylax crumenifer, the most prevalent amphistome species were selected at random for the present study. An indirect ELISA was performed to detect serum antibodies using somatic antigens of the fluke. Fifty one samples were found to be seropositive. Similarly, ELISA was employed to detect coproantigens in faecal supernatants of the same cattle using rabbit hyper immune serum against the somatic fluke antigens. Seventy four samples were found to contain detectable amounts of coproantigens indicating a sensitivity of 74 per cent for the test. The results indicate that coproantigen detection which revealed a higher sensitivity than that of serum antibodies by ELISA is reasonable and practical for the detection of amphistome infections in bovines.
Coproantigen, Indirect ELISA, Amphistomes