RESEARCH ARTICLES

Comparing ELISA using serum antibodies and coproantigens for detecting early amphistomosis
A Kandasamy and K Devada

Department of Veterinary Parasitology College of Veterinary and Animal Scineces Mannuthy 680 651, Thrissur, Kerala, India

Journal of Veterinary and Animal Sciences.2013. 44:35-37.

Open Access

 

 

Copyright: © 2013 A Kandasamy et.al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Abstract


Hundred cattle known to be infected with Gastrothylax crumenifer, the most prevalent amphistome species were selected at random for the present study. An indirect ELISA was performed to detect serum antibodies using somatic antigens of the fluke. Fifty one samples were found to be seropositive. Similarly, ELISA was employed to detect coproantigens in faecal supernatants of the same cattle using rabbit hyper immune serum against the somatic fluke antigens. Seventy four samples were found to contain detectable amounts of coproantigens indicating a sensitivity of 74 per cent for the test. The results indicate that coproantigen detection which revealed a higher sensitivity than that of serum antibodies by ELISA is reasonable and practical for the detection of amphistome infections in bovines.

Key words:

Coproantigen, Indirect ELISA, Amphistomes