Diagnosis of canine trypanosomosis by polymerase chain reaction
P V Tresamol, N P Usha, T V Aravindakshan, Reji Varghese and M R Saseendranath

Dept. of Veterinary Epidemiology & Preventive Medicine College of Veterinary and Animal Sciences, Mannuthy-680651, Thrissur, Kerala, India

Journal of Veterinary and Animal Sciences.2013. 44:66-69.

Open Access

Copyright: © 2013 Tresamol This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




A clinical case of trypanosomosis in a German Shepherd dog and its diagnosis by polymerase chain reaction(PCR) was described.The dog was presented with clinical signs such as pyrexia, bilateral corneal opacity, pale mucous membranes, emaciation and lymphadenopathy. Wet film and stained smear examination of peripheral blood revealed Trypanosoma evansi. Blood sample was also subjected to PCR for detection of parasitic DNA using the forward and reverse Kin primers, Kin1: 5’-GCG TTC AAA GAT TGG GCA ATG-3’ and KIN-2: 5’-CGC CCG AAA GTT CAC C-3’, which bind to an internal transcribed spacer region (ITS1). Polymerase chain reaction showed positive result and the amplicon size for T.evansi was 540bp. The case was successfully treated with a single dose of diminazine aceturate @10 mg/kg BW.

Keywords: Trypanosoma evansi, PCR, Kin Primers, Diminazine aceturate.