RESEARCH ARTICLES

CHARACTERISATION OI' IBD VIRAL PROTEINS IN SDS POLYACRYLAMIDE GEL ELECTROPHORESIS
M. Parthiball, V. Tliiagarajan

Four structural polypeptides of IBDV strain Cu-1 with molecular weights of 90 K (VP~), 41 K (VP,), 35 K (VP3) and 28 K (VP~) and the additional polypeptide VP~ (47 K) precursor of VP, were determined in SDS-PAGE (Dobos, 1979; Dobos et al., 1979). Hirai et al (1979) reported that seven structural polypeptides of IBDV strain GBF-1, consisted of two major and five minor polypeptides. Azad et al. (1987) reported that an Australian isolates type I IBDV was composed of two major structural proteins of molecular weight 37 K (VP,b) and 32 K (VP3} and three other proteins of molecular weight 92 K (VP~), 42 K (VP,a) and 27.5 K (VP~). Das and Ram {1994) showed that five different structural polypeptides of IBDV strain AP/ 81 with molecular weights of 75.8 K, 45 K, 40.7 K, 31.1 K and 27 K were detected in SDS-PAGE. Praveen et al. (1995) concluded that all the field isolates



Abstract