RESEARCH ARTICLES
Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species
Anvitha Hansoge, Sudheesh S Nair, M. V. Chinnu, M. K. Narayanan and K. D. John Martin
doi: https://doi.org/10.51966/jvas.
Journal of Veterinary and Animal Sciences.2021.52 (2):125-130.
Author Details
Anvitha Hansoge: M.V.Sc. scholar, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, India.
Sudheesh S Nair: Assistant Professor, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, India.
M.V. Chinnu: Assistant Professor, Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, India.
M.K. Narayanan: Professor, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, India.
K.D. John Martin: Professor and Head, Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, India.
Article History
Received: 08.12.2020, Accepted: 10.01.2021, Published online: 01.06.2021
Corresponding author: Sudheesh S Nair
e-mail: sudheeshnair@kvasu.ac.in, Ph. 7012782977
Citation: Anvitha, H., Nair, S.S., Chinnu,M.V., Narayanan, M.K. and John Martin, K.D. 2021. Fourier Transform Infrared Spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of Uroliths in caprine species. J. Vet. Anim. Sci. 52(2): 125-130. https://doi.org/10.51966/jvas.
Abstract
Pathophysiology of obstructive urolithiasis in goats are multifactorial involving management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treatment and prevention protocols. Four cases of chronic obstructive urolithiasis in male goats presented to University Veterinary Hospital were surgically managed by tube cystotomy. The calculi extracted from the bladder were subjected to Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) to identify the composition of the calculi. The infrared wavelength bandwidths of H-O-H stretching vibrations, H-O-H bending modes of vibrations of water molecules, N-H symmetric stretching vibrations, N-H bending vibration and N-H asymmetric bending vibration in NH4+ unit’s ionic phosphate were compared with the available reference spectrum of wavelength and were found to be identical and comparable with the standard infrared wavelength of struvite calculi.
Key words: Fourier transform infrared spectrophotometer, Attenuated total reflectance, Chronic obstructive urolithiasis, Goats
