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Scanning electron microscopy of ovary and oviduct of crossbred dairy cows with ovarian hypoplasia
Annie, V.R., Lucy, K.M., Ashok, N., Maya, S., Hiron, M.H. and Sathu, T.
doi: https://doi.org/10.51966/jvas.
Journal of Veterinary and Animal Sciences.2021.52 (2): 187-190.
Author Details
V.R. Annie: PhD Scholar, Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala- 680651,Kerala Veterinary and Animal Sciences University, India.
K.M. Lucy: Professor and Controller of Examinations, Directorate of Academics and Research, Kerala Veterinary and Animal Sciences University, India.
N. Ashok: Registrar, Kerala Veterinary and Animal Sciences University, India.
S. Maya: Professor and Head, Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala- 680651,Kerala Veterinary and Animal Sciences University, India.
M.H. Hiron: Assistant Professor, Department of Animal Reproduction Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala- 680651,Kerala Veterinary and Animal Sciences University, India.
T. Sathu: Assistant Professor, Department of Livestock Products Technology, Department of Veterinary Anatomy and Histology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala- 680651,Kerala Veterinary and Animal Sciences University, India.
Article History
Received: 06.01.2021 Accepted: 06.02.2021 Published online: 01.06.2021
Corresponding author: K.M. Lucy Email ID: lucy@kvasu.ac.in, Phone No. 9446408861
Citation: Annie, V.R., Lucy, K.M., Ashok, N., Maya, S., Hiron, M.H. and Sathu, T. 2021. Scanning electron microscopy of ovary and oviduct of crossbred dairy cows with ovarian hypoplasia. J. Vet. Anim. Sci. 52(2): 187-190. https://doi.org/10.51966/jvas.
Abstract
Hypoplasia of ovary is one of the major causes of infertility in dairy cattle, which is characterized by absence of oestrus cycle, affecting livestock productivity and economics to a great extent. This study was conducted on the female genitalia collected from 100 dairy cows/heifers from the Meat Technology Unit, Mannuthy which included six animals culled on account of factors other than infertility with normal genitalia (control group) and remaining animals with a known history of infertility. Two animals of 22 months and 24 months of age showed bilateral ovarian hypoplasia. The history revealed that the animals were healthy, but had not shown oestrus. Grossly ovaries of the first animal appeared as pink-coloured, small, wrinkled, flattened, elongated structures without any follicles or CL. Second animal showed inactive, small, flat, streak-like left ovary without any cyclical structures. But the right ovary showed a single large corpus haemorrhagicum on the caudal end. Then ovarian tissue was fixed in 2 percent gluteraldehyde in 0.1 M phosphate buffer at pH 7.3. Under scanning electron microscopy, the ovary exhibited an uneven surface with clefts and grooves. The surface cells lost their normal appearance without any microvilli. Large round smooth germ cells were also observed on the ovarian surface. In the oviduct, the mucosa was lined by nonciliated cells having rounded surface interspersed among shrunken cells. The epithelial cell surface was covered with bulbous processes. Ciliated cells were not observed in the present study. Ovarian abnormality is reported to be the main cause of altered morphology of the surface epithelial cells and change in the ultrastructure of oviductal mucosa.
Key words: Ovarian hypoplasia, Scanning Electron Microscopy, Dairy cows
