RESEARCH ARTICLES

BACTERIOLOGICAL EVALUATION OF ASEPTIC PROCEDURES ADOPTED FOR REPAIR OF COMPOUND FRACTURE OF RADIUS-ULNA IN DOGS
Karnail Mudahar, M.S. Oberoi and S.N. Sharma

Journal of Veterinary and Animal Sciences.1998. 29:53-58.

 

Open Access

 

Copyright: © 1998 Karnail Mudahar  et.al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Abstract


The study was conducted on 15 clinically healthy mongrel dogs. Experimentally created compound mid-shaft fracture of the radius-ulna was repaired by intramedullary pinning. Bacteriological samples were taken during various phases of surgery, from the skin of dogs, hands of surgeons, surgical packs, intramedullary pins, cutaneous wounds, operation theatre air, water and faeces. Haemoculturing was also done. Skin of dogs was found heavily contaminated with different types of micro-organisms. Its scrubbing with savlon (1 : 30) for 6 times reduced the microbial population by 99.9 % . Scrubbing of the hands by surgeons three times each with a detergent soap and with savlon (1:30) resulted insignificant reduction in number of micro-organisms. However, bacillus spp. and Staphylococcus spp. did not yield to scrubbing completely. Significant
spurt in bacterial count occurred, when the bandage slipped from the wound. Haemocultures taken upto 72 hrs. after fracture repair were bacteriologically sterile. There was no evidence of systemic infection, though nominal local infection was noticed. E. coli was isolated from faecal samples as well as from the wounds. Bacillus spp. and Staphylococcus spp. were isolated from both the scrubbed and unscrubbed skin of dogs, hands of surgeons, wound tissue, tip of intramedullary pin, water and operation theatre air.