Volume: 54 Issue: 1
Year: 2023, Page: 138-143, Doi: https://doi.org/10.51966/jvas.2023.54.1.138-143
Received: Oct. 27, 2022 Accepted: Dec. 20, 2022 Published: March 31, 2023
The present study was carried out with the objectives of assessing oxidative stress in dogs with chronic kidney disease (CKD) and evaluating response to treatment with N-acetyl cysteine (NAC). Dogs diagnosed with stage III CKD as per the guidelines of the International Renal Interest Society (IRIS) were included in the study. The animals were divided into two groups. Animals of one group were given standard therapy for CKD and the animals of the second group were administered NAC along with standard therapy. Oxidative stress parameters such as total antioxidant status (TAS), serum malondialdehyde (MDA) level and plasma glutathione peroxidase (GSH-Px) activity were studied. On the day of presentation, a significant increase in the mean values of serum MDA and TAS were observed in diseased animals compared to healthy animals, whereas a significant decline was noted in plasma GSH-Px activity. After treatment, a significant decline in serum MDA and TAS were recorded in animals of group II receiving NAC therapy. A significant increase in plasma glutathione GSH-Px activity was recorded in this group. N-acetyl cysteine therapy was found to be effective in the management of oxidative stress in dogs with chronic kidney disease.
Keywords: Chronic kidney disease, N-acetyl cysteine, oxidative stress
Bartges, J.W. 2012. Chronic kidney disease in dogs and cats. Vet. Clin. North Am. Small Anim. Pract. 42: 669-692.
Benzie, I.F. and Strain, J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem. 239: 70-76.
Brown, S.A. 2008. Oxidative stress and chronic kidney disease. Vet. Clin. North Am. Small Anim. Pract. 38: 157-166.
Costa-hong, V., Bortolotto, L.A., Jorgetti, V., Consolim- Colombo, F., Krieger, E.M. and Lima, J.J.G. 2009. Oxidative stress and endothelial dysfunction in chronic kidney disease. Arq. Bras. Cardiol. 92: 381–386.
Gultekin, M. and Voyvoda, H. 2017. Evaluation of oxidative status in dogs with anaemia. Med. Water. 73: 496-499.
Halfen, D.P., Caragelasco, D.S., Nogueira, J.P.S., Jeremias, J.T., Pedrinelli oba, P.M., Ruberti, B., Pontieri, C.F.F., Kogika, M.M. and Brunetto, M.A. 2020. Evaluation of electrolyte concentration and pro-inflammatory and oxidative status in dogs with advanced chronic kidney disease under dietary treatment. Toxins. 12: 1-11.
Katoch, A., Wadhwa, D.R. and Sharma, A. 2017. Epidemiological observations on canine renal disorders. Himachal J. Agric. Res. 43: 135-138.
Kaur, K.K., Allahbadia, G. and Mandeep. 2022. An update in the utilization of N-acetyl cysteine and vitamin C for tackling the oxidative stress in acute kidney injury secondary to robust sepsis- a systematic review. J. Clin. Nephrol. 6: 001-018.
Kogika M.M., Lustoza, M.D., Hagiwara, M.K., Caragelasco, D.S., Martorelli, C.R. and Mori, C.S. 2014. Evaluation of oxidative stress in the anaemia of dogs with chronic kidney disease. Vet. Clin. Path. 44: 70-78.
McMichael, M.A. 2007. Oxidative stress, antioxidants, and assessment of oxidative stress in dogs and cats. J. Am. Vet. Med. Ass. 231: 714-720.
Meagher, E.A. and FitzGerald, G.A. 2000. Indices of lipid peroxidation in vivo: strengths and limitations. Free Radic. Biol. Med. 28: 1745-1750.
Paglia, D.E. and Valentine, W.N. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70: 158-169.
Pleban, P.A., Munyani, A. and Beachum, J. 1982. Determination of selenium concentration and glutathione peroxidase activity in plasma and erythrocytes. Clin. Chem. 28: 311-316.
Polzin, D.J. 2011. Chronic kidney disease in small animals. Vet. Clin. North Am. Small. Anim. Pract. 41: 15-30.
Silva, A.C.R.A., Almeida, B.F.M., Soeiro, C.S., Ferreira, W.L., de Lima, V.M.F. and Ciarlini, P.C. 2013. Oxidative stress, superoxide production and apoptosis of neutrophils in dogs with chronic kidney disease. Can. J. Vet. Res. 77: 136-141.
Small, D.M., Coombes, J.S., Bennett, N., Johnson, D.W. and Gobe, G.C. 2012. Oxidative stress, anti-oxidant therapies and chronic kidney disease. Nephrology. 17: 311-321.
Yagi, K. 1984. Methods in Enzymology. Academic press, Inc, Burlington, 768p.
Zel, M.K., Tazon, N. and Svete, A.N. 2014. Plasma and erythrocyte glutathione peroxidase activity, serum selenium concentration and plasma total antioxidant capacity in cats with IRIS stages I-IV chronic kidney disease. J. Vet. Intern. Med. 28: 130-136.
© 2023 Balakrishnan et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Balakrishnan,N., Madhavan Unny,N., Ambily,V.R., Usha,N.P and Uma,R. 2023. Antioxidant effect of N-acetyl cysteine in dogs with chronic kidney disease. J. Vet. Anim. Sci. 54(1):138-143
DOI: https://doi.org/10.51966/jvas.2023.54.1.138-143