Volume: 48 Issue: 1
Year: 2017, Page: 90-93,
Received: July 1, 2016 Accepted: July 4, 2016 Published: June 30, 2017
The aim of the present study was to quantify the Progesterone (P4) and Estradiol- 17β (E2) in follicular fluid (FF) of medium (Gp-I; 3-5mm diameter) and large (Gp-II; more than 5mm diameter) goat ovarian follicles, by enzymelinked immune sorbent assay (ELISA). Pooled samples (n=7) of FF from two groups of follicles were used for the study. The E2 concentrations (ng/mL) in FF of Gp-I and Gp-II follices were 12.08±2.78 and 22.08±1.97 respectively. The P4 concentrations (ng/mL) in FF of Gp-I and Gp-II follicles were 10.56±2.3 and 20.53±3.82 respectively. The E2 and P4 concentrations were significantly (p≤0.05) higher in the Gp-II follicles than the Gp-I follicles. Similarly, increase in P4 concentration in Gp-II follicles might be due to the production of P4 in the synthetic pathway of follicular production of E2
Keywords: Progesterone, Estradiol-17β and Follicular Fluid
Berisha, B., Schams, D., Kosmann, M., Amselgruber, W. and Einspanier, R. 2000. Expression and tissue concentration of vascular endothelial growth factor, its receptors, and localization in the bovine corpus luteum during estrous cycle and pregnancy. Biol. Reprod. 63: 1106-1114.
Carpintero, N.L., Suárez, O.A., Mangas,C.C., Varea, C.G. and Rioja, G.R.2014. Follicular steroid hormones asmarkers of oocyte quality and oocyte development potential. J. Hum. Reprod. Sci. 7: 187-193.
Henderson, K.M., Mc Neilly, A.S. and Swanston, I.A. 1982. Gonadotrophin and steroid concentrations in bovine follicular fluid and their relationship to follicle size. J. Reprod. Fertil. 65: 467-473.
Ireland, J.J., Coulson, P.B. and Murphree, R.L. 1979. Follicular development during four stages of the estrous cycle of beef cattle. J. Anim. Sci. 49: 1261-1269.
Kalmath, G.P. and J.P. Ravindra. 2007. Mineral profiles of ovarian antral follicular fluid in buffaloes during follicle development. Indian J. Anim. Res. 41: 87-93.
Kruip, T.A. and Dieleman, S.J. 1985. Steroid hormone concentra-tions in the fluid of bovine follicles relative to size, quality and stage of the oestrus cycle. Theriogenology. 24: 395-408.
Mc Lachlan, R.I., Robertson, D.M., Healy, D.L., Burger, H.G. and Kretser, D.M. 1987. Circulating immunoreactive inhibin levels during the normal human menstrual cycle. J. Clin. Endocrinol. Metabol. 65: 954-961.
Senger, P.L. 2012. Pathways to pregnancy and parturition. (4th Ed.). Current conception, Washington, 373p.
Tungal, S.S., Swamy, M.N., Honnappa, T.G. and Bhaskaran, R. 2014. Ovarian Antral Follicular Dynamics and Follicular Fluid Composition in Non-descript Goats of Karnataka. Theriogenology. 4: 49-56.