Volume: 54 Issue: 2
Year: 2023, Page: 313-321, Doi: https://doi.org/10.51966/jvas.2023.54.2.313-321
Received: May 7, 2022 Accepted: Jan. 25, 2023 Published: June 30, 2023
The aim of this study was to detect the presence of carbapenem resistant Escherichia coli (E. coli) in cattle and pigs. Altogether, 100 samples were collected, 50 each from cattle and pigs including mastitic milk and faecal samples from healthy as well as diarrhoeic cattle and faecal samples and slaughter specimens from pigs. Bacteria were isolated from the samples and antibiotic sensitivity test was carried out. Eight isolates of E. coli were obtained from cattle and 15 isolates from pigs. The phenotypic resistance patterns against carbapenems (ertapenem, imipenem and meropenem) were analysed. In cattle, the four isolates from milk were observed to be sensitive to all the three carbapenems whereas, all the isolates obtained from faecal samples were resistant to all the three carbapenems. The four faecal and three intestinal isolates of E. coli from pigs showed sensitivity to all the three carbapenems. From a total of 10 faecal samples from pigs, four, three and one isolates showed resistance to ertapenem, imipenem and meropenem respectively. Among five isolates from intestine, one isolate showed resistance to ertapenem and imipenem and no isolate showed resistance to meropenem. Polymerase chain reaction (PCR) was carried out to find the presence of carbapenem resistance genes (blaSHV, blaCTX, blaTEM and blaNDM) in all the 23 isolates and the results revealed that seven (58.3 per cent) E. coli isolates carried blaSHV gene and four (33.3 per cent) contained blaCTX gene which encodes carbapenem resistant enzymes. Antibiogram using 14 commonly used antimicrobial agents revealed varied multidrug resistant patterns among the E. coli isolates.
Keywords: Antibiogram, carbapenem resistance, Escherichia coli, multidrug resistance
Baran, I. and Aksu, N. 2016. Phenotypic and genotypic characteristics of carbapenem-resistant Enterobacteriaceae in a tertiary-level reference hospital in Turkey. Ann. Clin. Microbiol. Antimicrob. 15: 20.
Bora, A. Hazarika, N.K., Shukla, S.K., Prasad, K.N., Sarma, J.B. and Ahmed, G. 2014. Prevalence of blaTEM, blaSHV and blaCTX-M genes in clinical isolates of Escherichia coli and Klebsiella pneumoniae from Northeast India. Indian J. Pathol. Microbiol. 57: 249-254.
Chandrasekaran, D., Venkatesan, P., Tirumurugaan, K.G., Nambi, A.P., Thirunavukkarasu, P.S., Kumanan, K., Vairamuthu, S. and Ramesh, S. 2014. Pattern of antibiotic resistant mastitis in dairy cows. Vet. Wld. 7: 389-394.
Chika, E., Charles, E., Ifeanyichukwu, I., Eucharia, O.C., Thaddeus, G., Angus, O.N., Malachy, U., Chika, E. and Ikegbunam, M.N. 2017. Detection of Metallo-β-lactamase (MBL) among Carbapenem-Resistant gram-negative bacteria from rectal swabs of cow and cloacae swabs of poultry birds. Ann. Med. Hlth. Sci. Res. 7: 51-56.
Clinical and Laboratory Standards Institute 2018. Performance standards for antimicrobial susceptibility testing; 28th international supplement M100-S21. Wayne, PA: CLSI.
Das, A., Guha, C., Biswas, U., Jana, P.S., Chatterjee, A. and Samanta, I. 2017. Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal. Vet. Wld. 10: 517-520.
Ejaz, H., Younas, S., Abosalif, K.O.A., Junaid, K., Alzahrani, B., Alsrhani, A., Abdalla, A.E., Ullah, M.I., Qamar, M.U. and Hamam, S.S.M. 2021. Molecular analysis of blaSHV, blaTEM and blaCTX-M in extended-spectrum β-lactamase producing Enterobacteriaceae recovered from fecal specimens of animals. PLoS One. 16: 1-9.
Enne, V.I., Cassar, C., Sprigings, K., Woodward, M.J. and Bennett, P.M. 2007. A high prevalence of antimicrobial resistant Escherichia coli isolated from pigs and a low prevalence of antimicrobial resistant E. Coli from cattle and sheep in Great Britain at slaughter. FEMS:Microbiol. Lett. 278: 193–199.
Gondal, A.J., Choudhry, N., Bukhari, H., Rizvi, Z. and Yasmin, N. 2022. Characterization of genomic diversity among carbapenem resistant Escherichia coli clinical isolates and antibacterial efficacy of silver nanoparticles from Pakistan. Microorganisms. 10: 1-12
Kagambega, A., Martikainen, O., Siitonen, A. Traore, A.S., Barro, N. And Haukka, K. 2012. Prevalence of diarrheagenic Escherichia coli virulence genes in the feces of slaughtered cattle, chickens, and pigs in Burkina Faso. Microbiol. Open. 1: 276–284.
Lakshmi, R. and Jayavardhanan, K.K. 2016. Isolation and identification of major causing bacteria from bovine mastitis. Int. J. Appl. Pure Sci. Agric. 2: 45-48.
Lalzampuia, H., Dutta, T.K., Warjri, I. and Chandra, R. 2013. PCR-based detection of extended-spectrum b-lactamases (blaCTX-M-1 and blaTEM) in Escherichia coli, Salmonella spp. And Klebsiellapneumoniae isolated from pigs in North Eastern India (Mizoram). Indian J. Microbiol. 53: 291–296.
Papp-Wallace, K.M., Endimiani, A., Taracila, M.A. and Bonomo, R.A. 2011. Mini review - Carbapenems: Past, Present and Future. Antimicrob. Agents Chemother. 55: 4943-4960.
Pruthvishree, B.S., Vinodh Kumar, O.R., Sinha, D.K., Malik, Y.P.S., Dubal, Z.B., Desingu, P.A., Shivakumar, M., Krishnaswamy, N. and Singh, B.R. 2016. Spatial molecular epidemiology of carbapenem-resistant and New Delhi metallo beta-lactamase (blaNDM)-producing Escherichia coli in the piglets of organized farms in India. J. Appl. Microbiol. 122: 1537-1546.
Quinn, P.J., Carter, M.E., Markey, B.K. and Carter, G.R. 1994. Clinical Veterinary Microbiology. Mosby Wolf, Spain. 648p.
Ranjan, R., Gupta, M.K. and Singh, K.K. 2011. Study of bovine mastitis in different climatic conditions in Jharkhand, India. Vet. Wld. 4: 205-208.
Revathi, M. 2018. Virulence gene profiling of Escherichia coli from bovine mastitis and therapeutic management of coliform mastitis. M. V. Sc. Thesis, Kerala Veterinary and Animal Sciences University, Pookode, 43p.
Rita, O.L., Linda, A.O., Augustina, A.S., Matilda, A.A. and Kwasi, O.D. 2021. Antimicrobial resistance of Escherichia coli from broilers, pigs and cattle in the Greater Kumasi metropolis, Ghana. Int. J. Microbiol. 2021: 02-04.
Sankar, S., Thresia, Bosewell, A. and Mini, M. 2021. Molecular detection of Carbapenem resistant gram negative bacterial isolates from dogs. Indian J. Anim. Res. B-4297: 1-5.
Shaikh, S., Fatima, J., Shakil, S., Rizvi, S.M.D. and Kamal, M.A. 2015. Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi J. Biol. Sci. 22: 90-101.
Singh, B.R., Vinodh Kumar, O.R., Sinha, D.K., Bhardwaj, M., Vadhana, P., Singh, S.V., Saraf, A., Pruthvishree, B.S. and Tamta, S. 2017. List of Carbapenem (Meropenem or Imipenem or Ertapenem) Resistant Bacteria Isolated at Epidemiology Laboratory of Indian Veterinary Research Institute, Izatnagar, India from April 2011 to August 2017 from Different Animal Sources and their Environment and Phenotypic and Genotypic characterization of the Isolates for Different Carbapenemases. Researchgate. [online]. Available: https://www.researchgate.net/publication/319872796 [September 2017].
Stein, R.A. and Katz, D.E. 2017. Escherichia coli, cattle and the propagation of disease. FEMS: Microbiol. Lett. 364: 1-11.
Sumathi, B.R., Veeregowda, B.M. and Gomes, A.R. 2008. Prevalence and antibiogram profile of bacterial isolates from clinical bovine mastitis. Vet. Wld. 1: 237-238.
Taggar, G., Rheman, M.A., Boerlin, P. and Diarra, M.S. 2020. Review: Molecular Epidemiology of Carbapenemases in Enterobacteriales from Humans, Animals, Food and the Environment. Antibiotics. 9: 1-21.
Tshitshi, L., Manganyi, M.C., Montso, P.K., Mbewe, M. and Ateba, C.N. 2020. Extended Spectrum Beta-Lactamase-Resistant Determinants among Carbapenem-Resistant Enterobacteriaceae from Beef Cattle in the North West Province, South Africa: A Critical Assessment of Their Possible Public Health Implications. Antibiotics. 9: 1-19.
van den Bogaard, A.E.J.M., London, N. and Stobberingh, E.E. 2000. Antimicrobial resistance in pig faecal samples from The Netherlands (five abattoirs) and Sweden. J. Antimicrob. Chemother. 45: 663-671.
Verma, H., Rawat, S., Sharma, N., Jaiswal, V. and Singh, R. 2017. Prevalence, bacterial etiology and antibiotic susceptibility pattern of bovine mastitis in Meerut. J. Ent. Zool. Stud. 6: 706-709.
Xavier, M., Janus, A., Deepa, P.M., Bipin, K.C., Habeeb, B.P. and Vijayakumar, K. 2020. Antibiogram of Escherichia coli isolates from faecal samples of neonatal calf diarrhoea in Wayanad district. J. Vet. Anim. Sci. 51: 119-122
© 2023 Sandeep et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sandeep, P.R., Ambily, R., Irshad.A and Joseph , S. 2023. Detection of carbapenem resistant Escherichia coli in cattle and pigs. J. Vet. Anim. Sci. 54(2):313-321
DOI: https://doi.org/10.51966/jvas.2023.54.2.313-321