Volume: 56 Issue: 2
Year: 2025, Page: 383-386, Doi: https://doi.org/10.51966/jvas.2025.56.2.383-386
Received: Sept. 25, 2024 Accepted: Feb. 15, 2025 Published: June 30, 2025
Mastitis is the inflammation of the mammary gland parenchyma and many microorganisms, mostly bacteria are associated with the condition. A study was conducted to assess whether mycoplasma is associated with cases of mastitis that are refractory to antibiotic treatment as the condition caused by the microbe is reported to be difficult to treat. A total of 71 milk samples were collected from antibiotic-resistant mastitic cows in the northern districts of Kerala. Total DNA was extracted from all the samples by phenol-chloroform method with prior sample lysis and subjected to Mycoplasma spp. and Mycoplasma bovis specific Polymerase Chain Reaction (PCR) and the amplicons obtained were sequenced. Mycoplasma spp. was detected in two (2.81 per cent) samples and no samples were positive for M. bovis. Sequencing of PCR amplicons revealed the presence of Mycoplasma hyorhinis in one of the samples. The identity of the Mycoplasma spp. in the other sample could not be conclusively proved as the nucleotide sequence obtained matched with that of M. bovis, M. arginini, and uncultured Mycoplasma spp. detected in nasal swabs of animals. Both the milk samples from which mycoplasma was detected were positive for multidrug-resistant gram positive and gram-negative bacteria and from one sample fungi could be isolated. From the results obtained, though mycoplasma was detected, it could not be proven that it is the sole cause of the condition. Also, the presence of mycoplasma is not wide spread in cases of refractory mastitis in northern Kerala.
Keywords: Mastitis, Mycoplasma, multi-drug-resistant bacteria, PCR
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Vishnu, P., Ravishankar, C., Rajasekhar, R., Sumod, K., Deepa, P.M., Janus, A., Sulficar, S., Pramod, S., Sooraj,
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