Journal of Veterinary and Animal Sciences

Volume: 54 Issue: 4

  • Open Access
  • Research Article

Effect of post-thaw supplementation of seminal plasma to the cryopreserved spermatozoa of Malabari bucks on in vivo fertility

K. Lekshmi Bhai1, B. Bibin Becha2*, C. Jayakumar3, Hiron M. Harshan2, M. Shynu4, Thirupathy Venkatachalapathy5 and K.A. Bindu6

1. PhD Scholar, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
2. Associate Professor,Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
3. Associate Professor and Head, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
4. Professor, Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651
Kerala Veterinary and Animal Sciences University, Kerala, India
5. Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651
Kerala Veterinary and Animal Sciences University, Kerala, India
6. Professor and Head, KVASU Goat and Sheep farm, Mannuthy

*Corresponding author: [email protected], Ph. 9447971872

Year: 2023, Page: 994-999, Doi: https://doi.org/10.51966/jvas.2023.54.4.994-999

Received: June 16, 2023 Accepted: July 12, 2023 Published: Dec. 31, 2023

Abstract

The study was conducted to assess the effect of post-thaw supplementation of seminal plasma to the cryopreserved spermatozoa of Malabari bucks on in vivo fertility. The ejaculates collected from Malabari bucks of good (n=3) and poor (n=3) semen freezability were pooled separately and cryopreserved. After thawing, seminal plasma from good semen freezability bucks was supplemented in such a way that group I (GFSP) included thawed semen of good freezability with supplementation, group II (GF) included thawed semen of good freezability without supplementation, group III (PFSP) included thawed semen of poor freezability with supplementation and group IV (PF) included thawed semen of poor freezability without supplementation. After a period of incubation for 10 min. at 37°C, the semen from each group was used for transcervical insemination of Malabari does of proven fertility. The results showed that there was a significant improvement in the conception rate with post-thaw supplementation. Percent of animals kidded also differed significantly in the four groups. But, prolificacy did not differ in GF and GFSP

Keywords: Post-thaw supplementation, seminal plasma, cryopreserved, Malabari bucks, conception rate

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Cite this article

Lekshmi, B.K., Becha, B.B., Jayakumar, C., Harshan, H.M., Shynu, M., Venkatachalapathy, R.T. and Bindu, K.A. 2023. Effect of post-thaw supplementation of seminal plasma to the cryopreserved spermatozoa of Malabari bucks on in vivo fertility. J. Vet. Anim. Sci. 54(4):994-999
DOI: https://doi.org/10.51966/jvas.2023.54.4.994-999

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