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Journal of Veterinary and Animal Sciences

Volume: 52 Issue: 2

  • Open Access
  • Research Article

Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species

Anvitha Hansoge1*, Sudheesh S Nair1, M.V. Chinnu2, M.K. Narayanan1 and K.D. John Martin1.

1. Department of Veterinary Surgery and Radiology

2. Department of Veterinary Biochemistry

College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, India

*Corresponding author: email : [email protected] Ph. 9590125001

Year: 2021, Page: 125-130, Doi: https://doi.org/10.51966/jvas.2021.52.2.125-130

Received: Dec. 8, 2020 Accepted: Jan. 10, 2021 Published: June 1, 2021

Abstract

Pathophysiology of obstructive urolithiasis in goats are multifactorial involving management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treatment and prevention protocols. Four cases of chronic obstructive urolithiasis in male goats presented to University Veterinary Hospital were surgically managed by tube cystotomy. The calculi extracted from the bladder were subjected to Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) to identify the composition of the calculi.The infrared wavelength bandwidths of H-O-H stretching vibrations, H-O-H bending modes of vibrations of water molecules, N-H symmetric stretching vibrations, N-H bending vibration and N-H asymmetric bending vibration in NH4+ unit’s ionic phosphate were compared with the available reference spectrum of wavelength and were found to be identical and comparable with the standard infrared wavelength of struvite calculi.

Keywords: Fourier transform infrared spectrophotometer, Attenuated total reflectance, Chronic obstructive urolithiasis, Goats

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Cite this article

Anvitha, H., Nair, S.S., Chinnu,M.V., Narayanan, M.K. and John Martin, K.D. 2021. Fourier Transform Infrared Spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of Uroliths in caprine species. J. Vet. Anim. Sci. 52(2): 125-130.

DOI: https://doi.org/10.51966/jvas.2021.52.2.125-130

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