Volume: 57 Issue: 1
Year: 2026, Page: 140-145, Doi: https://doi.org/10.51966/jvas.2026.57.1.140-145
Received: Oct. 6, 2025 Accepted: Nov. 14, 2025 Published: March 31, 2026
Leptospirosis is a widespread zoonotic disease of global importance caused by pathogenic species of Leptospira, affecting a wide range of animals including dogs. In dogs, leptospirosis remains a significant infectious disease characterised by multisystemic involvement posing both veterinary and public health challenges. Blood and serum samples were collected from a total of 65 dogs showing clinical signs suggestive of leptospirosis—including myalgia, haematuria, icterus, and pyrexia. Out of 65 dogs, 21 dogs confirmed as positive for leptospirosis on real-time PCR. Among the positive animals, haematological findings showed leucocytosis, neutrophilia, monocytosis, lymphocytosis, thrombocytopenia, along with decreased haemoglobin levels, haematocrit and total RBC count. Serum biochemical evaluation indicated increased levels of alkaline phosphatase, serum albumin, and both direct and indirect bilirubin, as well as elevated serum creatinine and blood urea nitrogen. These findings highlight that in association with clinical presentation and epidemiological information, haemato-biochemical alterations can serve as tool for tentative diagnosis as well as prognosis indicator.
Keywords: Leptospirosis, haemato-biochemical, bilirubin, creatinine, real time PCR
Abdullathief, K. A., Usha, N. P., Ajithkumar, S., Alex, P. C., & Joseph, S. (2018). Clinico-pathological studies on leptospirosis in dogs in Thrissur. Journal of Veterinary and Animal Sciences, 49, 9–13.
Alikhani, A., Mahmoodi, K., Delavaryan, L., Salehifar, E., Rafiei, A. R., & Sadeghi Zavare, M. (2022). Can cytokine response play a role in the treatment of fatal leptospirosis? Caspian Journal of Internal Medicine, 13, 343–348.
Andrade, M. C., Oliveira, L. B., Santos, Á. F., Moreira, M. V., Pierezan, F., & Ecco, R. (2020). Differential diagnoses in 83 dogs with icterus. Pesquisa Veterinária Brasileira, 40, 451–465.
Barry, M., Wisnewski, A. V., Matthias, M. A., Inouye, S. K., & Vinetz, J. M. (2006). Suburban leptospirosis: Atypical lymphocytosis and gamma-delta T cell response. Clinical Infectious Diseases, 43, 1304–1307.
Bovens, C., Fews, D., & Cogan, T. A. (2014). Leptospirosis and immune-mediated haemolytic anaemia in a dog. Veterinary Record Case Reports, 2, e000065.
Chou, L. F., Yang, H. Y., Hung, C. C., Tian, Y. C., Hsu, S. H., & Yang, C. W. (2023). Leptospirosis kidney disease: Evolution from acute to chronic kidney disease. Biomedical Journal, 46, Article 100595.
Durán-Galea, A., Cristóbal-Verdejo, J. I., Macías-García, B., Nicolás-Barceló, P., Barrera-Chacón, R., Ruiz-Tapia, P., Zaragoza-Bayle, M. C., & Duque-Carrasco, F. J. (2024). Determination of neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and systemic immune-inflammation index in dogs with leptospirosis. Veterinary Research Communications, 48, 4105–4111.
Howard, M. (2024). Acute kidney injury and liver disease in an American bulldog with suspected leptospirosis. Canadian Veterinary Journal, 65, 385–388.
Hoskins, J. D. (2004). Interpreting protein concentrations takes investigative work. DVM360. https://www.dvm360.com/view/interpreting-protein-concentrations-takes-investigate-work
Joseph, S., Mini, M., Sriram, V. K., Ambily, R., Aravindakshan, T., & Ajithkumar, S. (2018). Evaluation of real-time PCR, MAT and recombinant LipL32 based ELISA for the diagnosis of canine leptospirosis in a disease endemic South Indian state, Kerala. Turkish Journal of Veterinary and Animal Sciences, 42(3), 191–197.
Klosowski, M. L., & Bohn, A. A. (2023). Microscopic detection of Leptospira bacterial organisms in urine sediment from a young dog with leptospirosis and a review of the pathobiology and diagnosis of canine leptospirosis. Veterinary Clinical Pathology, 52, 112–118.
Knöpfler, S., Mayer-Scholl, A., Luge, E., Klopfleisch, R., Gruber, A. D., Nöckler, K., & Kohn, B. (2017). Evaluation of clinical, laboratory, imaging findings and outcome in 99 dogs with leptospirosis. Journal of Small Animal Practice, 58, 582–588.
Lees, G. E., & van Dongen, A. M. (2013). Consensus recommendations for the diagnostic investigation of dogs with suspected glomerular disease. Journal of Veterinary Internal Medicine, 27, 19–26.
Narayanavari, S. A., Sritharan, M., Haake, D. A., & Matsunaga, J. (2012). Multiple leptospiral sphingomyelinases (or are there?). Microbiology, 158, 1137–1146.
Novak, A., Pupo, E., Van’t Veld, E., Rutten, V. P. M. G., Broere, F., & Sloots, A. (2022). Activation of canine, mouse and human TLR2 and TLR4 by inactivated Leptospira vaccine strains. Frontiers in Immunology, 13, Article 823058.
Papa, A., & Kotrotsiou, T. (2015). Cytokines in human leptospirosis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 109, 749–754.
Rahman, M. S. A., Khor, K. H., Khairani-Bejo, S., Lau, S. F., Mazlan, M., Roslan, M. A., Ajat, M. M. M., & Noor, M. A. M. (2023). TaqMan real-time PCR for detection of pathogenic Leptospira spp. in canine clinical samples. Journal of Veterinary Research, 67(2), 187–195.
Schuller, S., Francey, T., Hartmann, K., Hugonnard, M., Kohn, B., Nally, J. E., & Sykes, J. (2015). European consensus statement on leptospirosis in dogs and cats. Journal of Small Animal Practice, 56, 159–179.
Sulistiawati, E., Putri, Z. U., Sajuthi, C. K., Sajuthi, D., & Oktaviani, N. (2022). Canine leptospirosis identification in small animal practice. Jurnal Kedokteran Hewan, 16, 1–5.
Sykes, J. E., Francey, T., Schuller, S., Stoddard, R. A., Cowgill, L. D., & Moore, G. E. (2023). Updated ACVIM consensus statement on leptospirosis in dogs. Journal of Veterinary Internal Medicine, 37, 1966–1982.
Tansakul, M., Sawangjai, P., Bunsupawong, P., Ketkan, O., Thongdee, M., Chaichoen, K., & Sakcamduang, W. (2024). Survival outcomes, low awareness and the challenge of neglected leptospirosis in dogs. Open Veterinary Journal, 14, 2368–2375.
Umesha, S., Chandan, S., Gopinath, S. M., & Shareef, M. I. (2014). Detection of Leptospira in canine samples using polymerase chain reaction based on LipL32 gene and biochemical profiles in canine leptospirosis. Journal of Cell Science and Molecular Biology, 1, 105.
Vieira, M. L., & Nascimento, A. L. T. O. (2020). Virulent Leptospira interrogans induce cytotoxic effects in human platelets in vitro through direct interactions. Frontiers in Microbiology, 11, Article 572972.
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