Volume: 56 Issue: 2
Year: 2025, Page: 280-284, Doi: https://doi.org/10.51966/jvas.2025.56.2.280-284
Received: Dec. 10, 2024 Accepted: Feb. 3, 2025 Published: June 30, 2025
This study aimed to investigate the alterations in the sperm quality parameters across high and low freezability semen samples of Malabari bucks during post-extension and pre-freeze stages of cryopreservation. A total of 32 semen ejaculates with at least 80 per cent initial progressive motility were selected, categorising four bucks as high freezability and four as low freezability based on post-thaw characteristics. Significant variation was found in progressive motility at the pre-freeze stage, with high freezability samples showing a markedly higher motility (68.25 ± 1.02 %) compared to low freezability samples (58.50 ± 1.0 %), indicating that motility is closely linked to freezability. Sperm viability was higher in the high freezability group post-extension (91.2 ± 0.56 %) and pre-freeze (79.70 ± 0.60 %) than in the low freezability group (91.00 ± 0.79 % and 78.85 ± 1.09 %, p < 0.05). Sperm abnormalities increased significantly from post-extension (high: 1.45 ± 0.25 %; low: 2.50 ± 0.11 %) to pre-freeze stages (high: 3.55 ± 0.34 %; low: 04.98 ± 0.36 %, p < 0.05). Acrosome integrity was consistently higher in the high freezability group across fresh (90.00 ± 0.49 %) and pre-freeze (82.91 ± 0.59 %) semen samples, compared to the low freezability group (87.20 ± 0.95 % and 78.58 ± 0.87 %, p < 0.05). Spermatozoa DNA integrity was significantly higher (p < 0.01) in high freezability samples (77.21 ± 0.86 %) compared to low freezability samples (67.95 ± 0.70 %) after equilibration. Pre-freeze (equilibration) phase effects on these parameters were evident, as demonstrated by the decline in motility, viability, and acrosome integrity between post-extension and pre-freeze stages. These findings suggested that selecting bucks based on freezability could improve semen quality, with tailored protocols necessary to address the unique requirements of low freezability bucks. The study also found a significant differences in the attributes of high and low freezable buck semen at the post-equilibration stage.
Keywords: Sperm attributes, freezability, fresh, pre-freeze, equilibration
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© 2025 Vigneshwaran et al. This is an open access article distributed under the terms of the Creative Commons Attribution
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Vigneshwaran, B., Leeba Chacko, Promod, K., Renjith Sebastain, Magnus Paul K., Aslam, M., Anupam Arun.
2024. Impact of equilibration on sperm quality in Malabari buck semen: A comparative study of different freezability
J. Vet. Anim. Sci. 56 (2):280-284