Volume: 55 Issue: 4
Year: 2024, Page: 721-726, Doi: https://doi.org/10.51966/jvas.2024.55.4.721-726
Received: Aug. 8, 2024 Accepted: Sept. 20, 2024 Published: Dec. 31, 2024
The purpose of this study was to investigate the occurrence of multi-drug resistant non-typhoidal Salmonella
spp. (MDR-NTS) in Wayanad district, Kerala. Initially, the cloacal swabs (n = 31) collected from different broiler farms in
the Wayanad district were subjected to isolation and identification of Salmonella spp. and confirmation by polymerase
chain reaction (PCR). A total of 24 (77.42 %) Salmonella spp. isolates were recovered by isolation and identification
in selective media and confirmed by PCR assay. Out of the 24 confirmed isolates, 20 (83.33 %) were confirmed to be
NTS strains, of which 2 (8.33 %) belonged to S. Typhimurium and 18 (75 %) were S. Enteritidis. Later, the antibiotic
susceptibility testing was carried out by a disc diffusion assay using 8 different classes of antibiotics. Of the 24 isolates
tested, 16 (66.67 %) were resistant to more than 4 classes of antibiotics, 10 (41.67 %) were resistant to more than or
equal to 6 classes of antibiotics, and 2 (8.33 %) were found to produce extended-spectrum beta-lactamase. Out of the 24
Salmonella spp. isolated, 21 (87.5 %) were confirmed to be MDR in nature, while 18 NTS strains exhibited MDR pattern.
The occurrence of MDR-NTS strains in broilers necessitates strict biosecurity measures to be adopted to reduce disease
incidence and avoid indiscriminate use of antibiotics in broiler farms to safeguard public health.
Keywords: Antibiotic, broiler chicken, multi-drug resistance, non-typhoidal Salmonella
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© 2024 Aswathi et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in
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Raghavendra, Aswathi, P.B., Jess, V., Reshma, M.M., Muneer, K.A., Prasoon, S. and Beena, J.C. 2024.
Isolation and characterisation of multi-drug resistant non-typhoidal Salmonella spp. from cloacal samples of broilers in
the Wayanad district. J. Vet. J. Vet. Anim. Sci. 55 (4):721-726