Journal of Veterinary and Animal Sciences

Volume: 54 Issue: 4

  • Open Access
  • Research Article

Mast cells as prognostic indicator in spontaneous canine mammary carcinomas

K. Krithiga1, Arathi Rajan2, Geetu Rose Varghese2, R. L. Neetha2, Neethu Krishnan2, Dipyaman Patra2, Arathy V. Warrier2 and Priya Srinivas3*

1. Assistant Professor, Bioscience Research and Training Centre, Thonnakkal,
Thiruvananthapuram-695 317. 
2. PhD scholar, CRP-6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram
3. Scientist G, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram
* Corresponding author: [email protected], Ph. 9447024097

Year: 2023, Page: 1005-1009, Doi: https://doi.org/10.51966/jvas.2023.54.4.1005-1009

Received: April 17, 2023 Accepted: July 13, 2023 Published: Dec. 31, 2023

Abstract

Tumour micro-environment is essential to nourish the cancer stem cells (CSC) which are said to be the major cause to initiate recurrence/ metastasis. Though there are many players, mast cells are said to play a key role in tumour metastasis. A cohort of twenty canine mammary carcinomas (CMC) was analysed for the presence of intra-tumoral and peri-tumoral mast cells, corelating it with the progression of the disease and exploring the possibility of finding a prognostic indicator. The mast cell density in the recurrent and non-recurrent CMC cases was examined with toluidine-blue staining. It could be appreciated that there is a significant difference between
the intra-tumoral mast cell count/density in malignant recurrent and non-recurrent carcinomas. However, such a difference could not be appreciated with the peri-tumoral tissues in both groups. The malignant recurrent carcinomas had less than 10 mast cells/ hpf, on an average from ten different fields, in the intra-tumoral tissues. Survival analysis carried out revealed that the CMC cases with less than 10 mast cells / hpf had a mean survival time of 24 months. This is the first report where the mast cells were studied for prognostic implications in CMC cases of Kerala. The potential role of these cells in the tumour micro-environment needs further investigation and may open avenues that lead to the identification of anti-oncogenic targets.

Keywords: Mast cells, canine mammary tumour, prognosis

References

Aller, M.-A., Arias, A., Arias, J.-I., and Arias, J. 2019. Carcinogenesis: the cancer cell–mast cell connection. Inflamm. Res. 68: 103-116. Available: doi:10.1007/s00011-018-1201-4 [02 March 2023].

Ariyarathna, H., Thomson, N., Aberdein, D. and Munday, J. S. 2020. Low Stromal Mast Cell Density in Canine Mammary Gland Tumours Predicts a Poor Prognosis. J. Comp. Pathology, 175: 29-38. Available: https://doi.org/10.1016/j.jcpa.2019.12.004 [02 March 2023].

Derakhshani, A., Vahidian, F., Alihasanzadeh, M., Mokhtarzadeh, A., Nezhad, L. P. and  
            Baradaran, B. 2019. Mast cells: A double-edged sword in cancer. Immunology
           Letters.
209: 28-35. Available:
https://doi.org/10.1016/j.imlet.2019.03.011[02 March
           2023].

Glajcar, A., Szpor, J., Pacek, A., Tyrak, K. E., Chan, F., Streb, J., Hodorowicz-Zaniewska, D. and Okoń, K. 2017. The relationship between breast cancer molecular subtypes and mast cell populations in tumor microenvironment. Virchows Arch. 470 (5) : 505-515. Available: doi:10.1007/s00428-017-2103-5 [02 March 2023].

Keser, S.H., Kandemir, N.O., Ece, D., Gecmen, G.G., Gul, A. E., Barisik, N. O., Sensu, S., Buyukuysal, C. and Barut, F. 2017. Kaohsiung J Med. Sci. 33:171-180.

Komi, D. E. A. and Redegeld, F. A. 2020. Role of Mast Cells in Shaping the Tumor Microenvironment. Clinical Reviews in Allergy & Immunology. 58: 313-325. Available: doi:10.1007/s12016-019-08753-w [02 March 2023].

Sakalauskaite, S., Riskeviciene, V., Sengaut, J. and Nomeda Juodziukyniene, N. 2022. Association of mast cell density, microvascular density and endothelial area with clinicopathological parameters and prognosis in canine mammary gland carcinomas. Acta. Vet. Scand. 64: 14. Available: https://doi.org/10.1186/s13028-022-00633-2 [15 March 2023].

Suvarna, K., Layton, C. and Bancroft, J. D. 2018. Bancroft’s Theory and Practice
           of Histological Techniques
. (8th Ed.). Elsevier Health Sciences, Churchill,
           584p.

Walcher, L., Kistenmacher, A.K., Suo, H., Kitte, R., Dluckzek, S., Straub, A.,   
         Blaudszun, A.R., Yevsa, T., Fricke, S. and Kossatz-Boehlert. U. 2020. Cancer Stem    
         Cells Origins and Biomarkers: Perspectives for Targeted Personalized Therapies.
         Front. Immunol.
Available: https://doi.org/10.3389/fimmu.2020.01280 [02 March
         2023].

Cite this article

Krithiga, K., Rajan, A., Varghese, G.R., Neetha, R.L., Krishnan, N., Patra, D., Warrier, A.V. and Srinivas, P. 2023. Mast cells as prognostic indicator in spontaneous canine mammary carcinomas. J. Vet. Anim. Sci. 54(4):1005-1009
DOI: https://doi.org/10.51966/jvas.2023.54.4.1005-1009

Views
30
Downloads
25
Citations