Journal of Veterinary and Animal Sciences

Volume: 54 Issue: 4

  • Open Access
  • Research Article

Pathological investigation and viral antigen distribution of postweaning multisystemic wasting syndrome in association with porcine parvovirus infection of pigs in Kerala, Southern India

R. Sairam1, B. Dhanush Krishna2*, K. Krithiga3, P.M. Priya4, R. Anoopraj5, Chintu Ravishankar6 and I.S. Sajitha7

1. M.V.Sc scholar, Department of Veterinary Pathology College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651
Kerala Veterinary and Animal Sciences University Kerala, India
2. Assistant Professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences,
Pookode, Wayanad-673 576.
3. Assistant Professor, Bioscience Research and Training Centre, Thiruvananthapuram-695017
4. Professor and Head, Department of Veterinary Microbiology, College of Veterinary and
Animal Sciences,Mannuthy-680 651
5. Assistant Professor
6. Professor and Head, Department of Veterinary Microbiology, College of Veterinary and
Animal Sciences, Pookode, Wayanad-673 576
7. Associate Professor
*Corresponding email: [email protected], Ph.9497891394

Year: 2023, Page: 1022-1034, Doi: https://doi.org/10.51966/jvas.2023.54.4.1022-1034

Received: May 19, 2023 Accepted: Sept. 12, 2023 Published: Dec. 31, 2023

Abstract

Postweaning multisystemic wasting syndrome (PMWS) is one of the important emerging porcine circoviral associated disease (PCVAD) causing immense economic losses throughout the world producing mortality and morbidity mainly in post-weaned pigs. During the period from 2018-2019, a total of 50 pig carcasses from different parts of Kerala were screened for concurrent infections of porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2), followed by the establishment of PMWS. All the samples were subjected to polymerase chain reaction (PCR) initially followed by histopathology and immunohistochemistry (IHC). Phylogenetic analysis was performed for PPV using non-structural gene -1(NS1). The result of the molecular screening using polymerase chain reaction revealed only one sample to be positive for both PCV2 and PPV. The gross lesions noted were the non-collapsed voluminous lungs, necrotising lymphadenitis, interstitial nephritis, splenomegaly and fibrinous pericarditis. Histopathological examination revealed mild interstitial pneumonia, lymphoid depletion in periarteriolar lymphoid sheaths of spleen with focal areas of granulomatous inflammation, depletion of the germinal centre of the follicles in the lymph nodes, non-suppurative myocarditis and interstitial nephritis. Further, immunohistochemistry demonstrated PCV2 and PPV antigens in the affected tissues. Based on the clinical signs, histopathological lesions an  immunohistochemical demonstration of PCV2 antigen, the case was confirmed as PMWS in association with PPV. This report of PMWS in association with PPV confirms, probably for the first time in the Kerala state of India.

Keywords: Post weaning multisystemic disease, porcine circovirus 2, porcine parvo virus, immunohistochemistry

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Cite this article

Sairam, R., Krishna, B.D., Krithiga, K., Priya, P.M., Anoopraj, R., Ravishankar, C. and Sajitha, I.S. 2023. Pathological investigation and viral antigen distribution of postweaning multisystemic wasting syndrome in association with porcine parvovirus infection of pigs in Kerala, Southern India. J. Vet. Anim. Sci. 54(4):1022-1034
DOI: https://doi.org/10.51966/jvas.2023.54.4.1022-1034

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