Volume: 54 Issue: 1
Year: 2023, Page: 1-6, Doi: https://doi.org/10.51966/jvas.2023.54.1.1-6
Received: Aug. 3, 2021 Accepted: Sept. 13, 2021 Published: March 31, 2023
The present study was conducted to identify single nucleotide variations in exon 3 region of the bovine Toll-Like Receptor gene (TLR4) associated with somatic cell score (SCS) in crossbred cattle of Kerala using polymerase chain reaction-single stranded conformational polymorphisms (PCR-SSCP) technique. Blood samples were collected from 200 lactating crossbred cattle in different farms of Kerala Veterinary and Animal Sciences University (KVASU) and field centres of ICAR- FPT scheme, KVASU. Genomic DNA was isolated and polymorphisms were identified by SSCP analysis. The 231 bp fragment of exon 3 in TLR4 gene was found to polymorphic and two genotypes viz. CC and CD were obtained with frequencies 77 per cent and 23 per cent respectively. The frequencies of C and D allele in crossbred were found to be 0.88 and 0.12. On sequencing, C to T transition had led to one non-synonymous change at 2021th position of ORF. Cattle with CC genotype were associated with significantly lower SCS (p≤0.05) as compared to CD genotype. The association between TLR4 polymorphism and SCS described in this study suggests the possible role of SNP during mastitis infection. Thus, could be employed as a useful marker for genetic selection to promote mastitis resistance in dairy cattle.
Keywords: TLR4, PCR-SSCP, somatic cell score, crossbred cattle
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© 2023 Kiyevi et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Kiyevi,G.C., Jamuna,V., Tina,S., Anilkumar,K., Aravindakshan,T.V. and Radha,K. 2023. Polymorphism study of TLR4 gene in crossbred cattle of Kerala. J. Vet. Anim. Sci. 54(1):1-6
DOI: https://doi.org/10.51966/jvas.2023.54.1.1-6