Journal of Veterinary and Animal Sciences

Volume: 54 Issue: 4

  • Open Access
  • Research Article

Post-thaw quality of Malabari buck semen with different freezing resilience

K. Lekshmi Bhai1, B. Bibin Becha2*, C. Jayakumar3, Hiron, M. Harshan2, M. Shynu4 and R. Thirupathy Venkatachalapathy5

1. PhD scholar, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
2. Associate professor, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
3. Associate professor and head , Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
5. Professor, Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India
6. Professor, Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 680 651, Kerala Veterinary and Animal Sciences University, Kerala, India


*Corresponding author: [email protected], Ph. 9447971872

Year: 2023, Page: 980-987, Doi: https://doi.org/10.51966/jvas.2023.54.4.980-987

Received: April 4, 2023 Accepted: July 19, 2023 Published: Dec. 31, 2023

Abstract

The study was conducted to assess the difference in post-thaw quality parameters of Malabari buck spermatozoa of good and poor semen freezability. The ejaculates collected from Malabari bucks of good (n=3) and poor (n=3) semen freezability were pooled separately as group A and group B, respectively after the initial evaluation. The extended semen in each group was processed and cryopreserved by the conventional method of static vapour freezing. After thawing, the quality of semen in both groups was assessed in terms of progressive motility, viability, morphological abnormalities, acrosome integrity, functional membrane integrity and lipid peroxidation status (MDA) of spermatozoa. Results showed that there was no significant difference between the fresh semen characteristics of group A and B bucks except for concentration, progressive motility and functional membrane integrity of spermatozoa. But, post-thaw quality of groups A and B differed significantly in terms of progressive motility (48.89 ± 0.64 % vs 18.06 ± 1.08 %), viability (55.17 ± 0.57 % vs 26.33 ± 0.95 %), acrosome integrity (62.44 ± 0.62 % vs 33.42 ± 0.34 %), functional membrane integrity (47.00 ± 0.36 % vs 23.36 ± 0.63 %) and MDA concentration (7.83 ± 0.29 nmol/mL vs 13.95 ± 0.08 nmol/mL) whereas morphological abnormalities (2.11 ± 0.08 % vs 2.39 ± 0.09 %) did not differ significantly. The findings of the present study showed that even with similar fresh semen characteristics, spermatozoa from different Malabari bucks have varying degrees of cryosurvivability.

Keywords: Malabari buck spermatozoa, good semen freezability, poor semen freezability, freezing resilience, cryopreservation

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Cite this article

Lekshmi B.K., Becha, B.B., Jayakumar, C., Harshan, H.M., Shynu, M. and Venkatachalapathy, R.T. 2023. Post-thaw quality of Malabari buck semen with different freezing resilience. J. Vet. Anim. Sci. 54(4):980-987
DOI: https://doi.org/10.51966/jvas.2023.54.4.980-987

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