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Journal of Veterinary and Animal Sciences

Volume: 56 Issue: 2

  • Open Access
  • Research Article

Postmortem interval estimation in cats using vitreous potassium: Development of a regression model

F. Almas1, M. Pradeep1*, A. J. George1, B. D. Krishna2, S. S. Devi3, R. Rajasekhar4 and N.K. Mansoor5

1Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Pookode, Wayanad- 673 576,2Department
of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur- 608 651,3Bioscience Research
and Training Centre, Thonnakkal, Thiruvananthapuram- 695 317,4Department of Veterinary Microbiology, College of
Veterinary and Animal Sciences, Pookode, Wayanad- 673 576., Kerala Veterinary and Animal Sciences University, Kerala,
India, 5Department of Statistics, Sir Syed college Taliparamba, Kannur- 670 142, Kannur University, Kerala, India

Year: 2025, Page: 236-239, Doi: https://doi.org/10.51966/jvas.2025.56.2.236-239

Received: Sept. 30, 2024 Accepted: March 24, 2025 Published: June 30, 2025

Abstract

Accurate postmortem interval (PMI) estimation is critical in forensic investigations and in vetero-legal procedures. Even though vitreous humour potassium concentration has been studied previously in humans and dogs to estimate PMI, studies on cats are scanty. The present study investigated vitreous potassium concentrations in cat carcasses  different PMI and its potential for estimating PMI in cats. The cats with known PMI brought for post-mortem examination at the Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Pookode, Wayanad formed the study material. Vitreous humour was collected from 12 cat carcasses with PMIs ranging from one to fifty-two hours. Data including the age and sex of the cats and ambient temperature at the time of collection were recorded. Potassium concentration was quantified from clear supernatant collected after centrifugation of vitreous humour using the ionselective electrode method. Statistical analysis revealed a strong positive correlation between potassium concentration and PMI (r= 0.919, p < 0.01) with no significant influence of age, sex or ambient temperature. Regression analyses demonstrated that potassium concentration explained 84.5% of the variance in PMI with the power regression model demonstrating the best predictive accuracy (R² = 0.978). This study confirms that vitreous potassium concentration is a robust biomarker for PMI estimation in cats in early periods after death and provides a reliable regression model for forensic applications.

Keywords: Post-mortem interval, vitreous humour, potassium concentration, cat, forensic veterinary medicine, regression model

References

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Cite this article

Almas, F., Pradeep, M., George, A.J., Krishna, B.D., Devi, S.S., Rajasekhar, R., and Mansoor, N.K. 2025. Postmortem interval estimation in cats using vitreous potassium concentration Development of a regression model. J. Vet. Anim. Sci. 56 (2):236-239

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