Volume: 57 Issue: 1
Year: 2026, Page: 69-73, Doi: https://doi.org/10.51966/jvas.2026.57.1.69-73
Received: Sept. 11, 2025 Accepted: Dec. 15, 2025 Published: March 31, 2026
The development of species-specific protocols for platelet-rich plasma (PRP) preparation is critical for ensuring quality and reproducibility in veterinary regenerative medicine. This study aimed to standardise the preparation of homologous PRP from goat blood using a double centrifugation method and to validate its efficacy based on platelet enrichment and recovery. Venous blood was collected from bucks and baseline haematological parameters were assessed. A two-step centrifugation protocol was employed: a soft spin at 300×g for 5 min. followed by a hard spin at 700×g for 17 min. The resulting PRP was analysed for erythrocyte, leukocyte and platelet concentrations, and quantitative indices including platelet enrichment factor (PEF) and platelet recovery rate (PRR) were calculated. Compared with whole blood, PRP exhibited a marked higher concentration of platelet (1465.50 ± 124.42 ×10³/μL vs. 413.50 ± 24.95 ×10³/μL), with an average PEF of 3.74 ± 0.21 and PRR of 78.49 ± 3.94 per cent. Red and white blood cells were substantially reduced in PRP, confirming successful separation. The protocol provided effective platelet concentration while minimising contamination, although separation challenges due to the small size of goat platelets and erythrocytes were noted. These findings support the feasibility of standardised homologous PRP preparation in goats and highlight the need for further refinement to improve purity.
Keywords: Platelet-rich plasma, goat, double centrifugation, platelet enrichment
Bharti, D., Ajith, Y., Sharun, K., Banu, S. A., Kumar, A., Bhardwaj, A., Sidar, S. K., & Dhaleshwari. (2024). Therapeutic applications of canine platelets and their derivatives: A narrative review. Topics in Companion Animal Medicine, 58, 100840.
Byers, S. R., Kramer, J. W., Weiss, D. J., & Wardrop, K. J. (2010). Normal hematology of sheep and goats. In D. J. Weiss & K. J. Wardrop (Eds.), Schalm’s veterinary hematology (6th ed., pp. 836–842). Wiley-Blackwell.
Clemmons, R. M., Bliss, E. L., Dorsey-Lee, M. R., Seachord, C. L., & Meyers, K. M. (1983). Platelet function, size and yield in whole blood and in platelet-rich plasma prepared using differing centrifugation force and time in domestic and food-producing animals. Thrombosis and Haemostasis, 50, 838–843.
Dashore, S., Chouhan, K., Nanda, S., & Sharma, A. (2021). Preparation of platelet-rich plasma: National IADVL PRP taskforce recommendations. Indian Dermatology Online Journal, 12(Suppl. 1), S12–S23.
Dhurat, R., & Sukesh, M. (2014). Principles and methods of preparation of platelet-rich plasma: A review and author’s perspective. Journal of Cutaneous and Aesthetic Surgery, 7, 189–197.
do Amaral, R. J. F. C., da Silva, N. P., Haddad, N. F., Lopes, L. S., Ferreira, F. D., Filho, R. B., Cappelletti, P. A., de Mello, W., Cordeiro-Spinetti, E., & Balduino, A. (2016). Platelet-rich plasma obtained with different anticoagulants and their effect on platelet numbers and mesenchymal stromal cell behavior in vitro. Stem Cells International, 2016, 7414036.
Foster, T. E., Puskas, B. L., Mandelbaum, B. R., Gerhardt, M. B., & Rodeo, S. A. (2009). Platelet-rich plasma: From basic science to clinical applications. The American Journal of Sports Medicine, 37, 2259–2272.
Harrison, T. E., Bowler, J., Levins, T. N., Cheng, A. L., & Reeves, K. D. (2020). Platelet yield and yield consistency for six single-spin methods of platelet-rich plasma preparation. Platelets, 31, 661–666.
Hernandez-Corredor, L., Leon-Restrepo, S., Bustamante-Cano, J., Baez-Sandoval, G., & Jaramillo, X. (2020). Effect of the incorporation of platelet-rich plasma on the spermatozoa physiology of ram semen. Journal of Dairy, Veterinary & Animal Research, 9, 34–38.
Hoareau, G. L., Jandrey, K. E., Burges, J., Bremer, D., & Tablin, F. (2014). Comparison of the platelet-rich plasma and buffy coat protocols for preparation of canine platelet concentrates. Veterinary Clinical Pathology, 43, 513–518.
Jakfar, S., Ningsih, D. S., Lin, T. C., Chen, Z. Y., Lin, F. H., Gani, B. A., Syafriza, D., & Kusuma, H. (2024). Evaluation of a membrane filter and platelet-rich plasma product obtained from a prototype PRP kit based on a new cell-dimension separation method. AIP Advances, 14.
Menaka, R., & Singh, I. S. (2006). Cytomorphological studies on the blood cells of goat. Indian Veterinary Journal. http://www.indvetjournal.com
Okumo, T., Sato, A., Izukashi, K., Ohta, M., Oike, J., Yagura, S., Okuma, N., Koya, T., Sunagawa, M., & Kanzaki, K. (2023). Multifactorial comparative analysis of platelet-rich plasma and serum prepared using a commercially available centrifugation kit. Cureus, 15, e48918.
Philip, L. M. (2022). Autologous platelet-rich plasma for treatment of sole lesions and expression profile of associated biomarkers in dairy cattle (Ph.D. thesis, Kerala Veterinary and Animal Sciences University, Pookode).
Reji, V., Anoop, S., Syam, K. V., Sooryadas, S., Vasudevan, V. N., Varuna, P. P., Sudheesh, S. N., Deny, J., & Martin, J. K. D. (2025). Modified syringe technique to prepare platelet-rich plasma from rat blood: Quantitative assessment and evaluation of platelets stored at 4 °C for homologous application. Journal of Veterinary and Animal Sciences, 56(1), 13–19.
Salama, M. S., Shehabeldin, A. M., Ashour, M. A., Al-Ghadi, M. Q., Marghani, B. H., El-Kon, I., & Shukry, M. (2024). Effect of the addition of platelet-rich plasma to Boer buck semen on sperm quality and antioxidant activity before and after cryopreservation and in vivo fertility. Small Ruminant Research, 230, 107167.
Samadi, P., Sheykhhasan, M., & Khoshinani, H. M. (2019). The use of platelet-rich plasma in aesthetic and regenerative medicine: A comprehensive review. Aesthetic Plastic Surgery, 43, 803–814.
Stayt, J. (2022). Hematology of sheep and goats. In D. J. Weiss & K. J. Wardrop (Eds.), Schalm’s veterinary hematology (6th ed., pp. 1012–1018). Wiley-Blackwell.
© 2026 Murali et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Murali, A., Becha, B.B., Jayakumar, C., Harshan, H.M., Chandrasekhar, L. & Pratheesh, M.D. 2025. Preparation and validation of platelet rich plasma from goat blood. Journal of Veterinary and Animal Sciences 57 (1), 69-73 https://doi.org/10.51966/jvas.2026.57.1.69-73