Volume: 55 Issue: 4
Year: 2024, Page: 698-703, Doi: https://doi.org/10.51966/jvas.2024.55.4.698-703
Received: June 11, 2024 Accepted: Oct. 16, 2024 Published: Dec. 31, 2024
The current study was undertaken to analyse the cellular components and three-dimensional organisation of
“platelet rich fibrin” membrane (PRFM), an autologous blood derived biomaterial. Ten millilitres of whole blood collected
from six canine patients which was then centrifuged at 542.4G (3200 rpm for 10 minutes). The PRF clot thus obtained
was pressed to form the platelet rich fibrin membrane (PRFM). The structural analysis and cell composition of PRFM was
analysed using scanning electron microscopy (SEM) and histopathology. Evaluation with SEM revealed clearly organised
fibrin in parallel strands and the cells were embedded in this compact fibrin matrix. Histopathological examination showed
that the leukocyte cytoplasm stained a darker pink along with RBCs, the fibrin matrix seemed homogenous in light pink
and platelet aggregates were dark blue/violet. According to the study, the majority of the platelets and leukocytes from the
blood sample were present in the PRFM, and their distribution followed a characteristic three-dimensional pattern with
the fibrin strands.
Keywords: PRF, SEM, dogs, keratitis
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© 2024 Kumar et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original author and source are credited.
Kumar, A.S., Venugopal, S.K., Philip, L.M., Ramankutty, S., Martin, K.D.J. and Prasanna, K.S. 2024. Preparation
of canine platelet-rich fibrin membrane and its characterisation using light microscopy and scanning electron microscopy.
J. Vet. Anim. Sci. 55 (4):698-703